Evidence map›Paper›PMID 39192136›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2025

Protein Kinase R (PKR) as a Novel dsRNA Sensor in Antiviral Innate Immunity.

Huibin Yu, Dewi Megawati, Chunfu Zheng, Stefan Rothenburg

Erratum issuedAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
  4. Article
  5. Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Huibin YuDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.
Dewi MegawatiDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA.
Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.
Stefan RothenburgDepartment of Medical Microbiology and Immunology, School of Medicine, University of California, Davis, Davis, CA, USA. rothenburg@ucdavis.edu.

Funding

Molecular mechanisms and ramifications of horizontal gene transfer into poxvirusesR01AI146915 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ROTHENBURG, STEFAN · 2020 to 2024
$1.9M
Importance of Species-Specific Interactions of PKR with Poxvirus Inhibitors for Virus Replication and Host RangeR01AI114851 · NIAID · UNIVERSITY OF CALIFORNIA AT DAVIS · PI ROTHENBURG, STEFAN · 2015 to 2019
$1.8M
NIAID NIH HHS R01 AI114851NIAID NIH HHS R01 AI146915
6 · The paper itself

Abstract

Protein kinase R (PKR), a key double-stranded RNA (dsRNA)-activated sensor, is pivotal for cellular responses to diverse stimuli. This protocol delineates a comprehensive methodological framework employing single luciferase assays, yeast assays, immunoblot assays, and quantitative PCR (qPCR) to discern and validate PKR activities and their downstream impacts on NF-κB-activating signaling pathways. These methodologies furnish a systematic approach to unraveling the role of PKR as a dsRNA sensor and effector in antiviral innate immunity, enabling in-depth analyses of dsRNA sensor activities.

Indexed as

eIF-2 KinaseImmunity, InnateRNA, Double-StrandedAnimalsHumansNF-kappa BSignal TransductioneIF-2 KinaseNF-kappa BRNA, Double-StrandedeIF2α phosphorylationImmunoblotsNF-κB pro-inflammatory pathwayPKRPKR inhibitorqPCRRNA sensorSingle luciferase assayYeast assay

Identifiers

PMID39192136
PMCPMC13289686

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.