Cryo-Electron Microscopy in the Study of Antiviral Innate Immunity.

Yan Chen, Jingyu Wang, Chunfu Zheng, Zheng Liu

Abstract read

In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it

0cells of the map it votes in

0citing papers in PubMed

–field-weighted citation impact

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Yan Chen *School of Medicine, Shaoguan University, Shaoguan, Guangdong, China.

Jingyu Wang *Distinct HealthCare Medical Center, Changsha Section, Shenzhen, China.

Chunfu ZhengDepartment of Microbiology, Immunology and Infectious Diseases, University of Calgary, Calgary, AB, Canada.

Zheng LiuKobilka Institute of Innovative Drug Discovery, School of Medicine, the Chinese University of Hong Kong, Shenzhen, Guangdong, China. liuzheng@cuhk.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryo-electron microscopy is a powerful methodology in structural biology and has been broadly used in high-resolution structure determination for challenging samples, which are not readily available for traditional techniques. In particular, the strength of super macro-complexes and the lack of a need for crystals for cryo-EM make this technique feasible for the structural study of complexes involved in antiviral innate immunity. This chapter presents detailed information and experimental procedures of Cryo-EM for determining the structures of the complexes using STING as an example. The procedures included a sample quality check, high-resolution data acquisition, and image processing for Cryo-EM 3D structure determination.

Indexed as

Cryoelectron MicroscopyImmunity, InnateHumansImage Processing, Computer-AssistedImaging, Three-DimensionalMembrane ProteinsSTING ProteinMembrane ProteinsSTING1 protein, humanSTING ProteinBiological macro-complexCryo-electron microscopyStructure determinationThree dimensional structure

Identifiers

PMID39192129

DOI10.1016/j.jsb.2004.06.006

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.