ArticleScientific reports2024
Comparative metabolome variation in Brassica juncea different organs from two varieties as analyzed using SPME and GCMS techniques coupled to chemometrics.
Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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8 citing papers in PubMed.
- Systematic mass-spectrometry-guided metabolic fingerprinting elucidates diversity of specialized metabolites across the Brassicaceae.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Effects of cultivar and stir-frying on the volatile flavor characteristics of white and yellow mustard seeds.Food chemistry: X · 2026Article
- Unveiling the Biosynthetic Mechanisms of Dietary Fiber in the Leaves and Stems of Mustard (Journal of agricultural and food chemistry · 2026Article
- NMR- and MS-based multiplex approach for comparative metabolome profiling of Curcuma caesia versus curcuma longa rhizomes and in relation to in vitro biological effects.Scientific reports · 2025Article
- Comprehensive metabolites characterization of Alpinia katsumadai seeds via a multiplex approach of UHPLC-MS/MS and GC-MS techniques.Scientific reports · 2025Article
- Proteomic changes associated with health benefit properties in fresh and preserved mustard greens.Food chemistry. Molecular sciences · 2025Article
- GC-MS based nutritional and aroma profiling of date palm seeds collected from different Egyptian cultivars for valorization purposes.Scientific reports · 2025Article
- Multi-Omics Approaches Against Abiotic and Biotic Stress-A Review.Plants (Basel, Switzerland) · 2025Review
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3 authors.
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Abstract
Indian mustard (Brassica juncea; Brassicaceae) is an edible, oilseeds-yielding crop widely consumed as a food spice owing to its richness in nutrients with several health benefits. The current study aims to dissect the B. juncea metabolome heterogeneity among its different organs including leaf, stem, flower, and seed. Moreover, assessing the metabolome differences between two different varieties RH-725 and RH-761 grown at the same conditions. Gas chromatography-mass spectrometry (GC-MS) post-silylation was used to dissect the composition of nutrient metabolites coupled to multivariate data analysis. Variation in sulphur aglycones was measured using headspace-solid phase-microextraction HS-SPME coupled to GC-MS. A total of 101 nutrient metabolites were identified with the abundance of sugars represented by monosaccharides in all organs, except for seeds which were enriched in disaccharides (sucrose). α-Linolenic acid was detected as a marker fatty acid in leaf from RH-725 at 12.5 µg/mg. Malic acid was detected as a significant variant metabolite between the two varieties as detected in the leaf from the RH-725 variety at ca. 128.2 µg/mg compared to traces in RH-761. 7 Volatile sulphur compounds were detected at comparable levels in RH-725 and RH-761, with 3-butenyl isothiocyanate was the most abundant at 0.8-2 ng/mg.
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