Evidence map›Paper›PMID 39190281›Full record

ArticleActa parasitologica2024

Detection of Leishmania RNA Virus 2 (LRV2) among Clinical Isolates of Leishmania Major in Four Endemic Regions of Iran.

Samaneh Mazaherifar, Saiedeh Erfanian, Kavous Solhjoo, Abazar Roustazadeh, Mohammad Darayesh, Ali Taghipour, Shahab Falahi, Azra Kenarkoohi, Milad Badri, Seyede Manizhe Heidarnejadi and 2 more

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Article in Acta parasitologica, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Journal of parasitology research · 2026
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Samaneh MazaherifarZoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Saiedeh ErfanianResearch Center for NonCommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
Kavous SolhjooZoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Abazar RoustazadehResearch Center for NonCommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
Mohammad DarayeshResearch Center for NonCommunicable Diseases, Jahrom University of Medical Sciences, Jahrom, Iran.
Ali TaghipourZoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Shahab FalahiZoonotic Diseases Research Center, Ilam University of Medical Sciences, Ilam, Iran.
Azra KenarkoohiDepartment of Microbiology, Ilam University of Medical Sciences, Ilam, Iran.
Milad BadriMedical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Seyede Manizhe HeidarnejadiShoushtar Faculty of Medical Sciences, Shoushtar, Iran.
Sima RastiDepartment of Parasitology and Mycology and Infectious Diseases Research Center, Faculty of Medicine, Kashan University of Medical Sciences, Kashan, IR, Iran.
Amir AbdoliZoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran. a.abdoli25@gmail.com.ORCID http://orcid.org/0000-0003-4326-4586

Funding

Jahrom University of Medical Sciences 99000159National Institute for Medical Research Development 978507
6 · The paper itself

Abstract

purposeLeishmania RNA viruses (LRV) are double-stranded RNA viruses (dsRNA viruses) that play a role in the pathogenesis of Leishmania parasites. Cutaneous leishmaniasis (CL) is endemic in various parts of Iran. Our aimed was to investigate presence of LRV among the Leishmania major isolates in four endemic regions of Iran.

methodsIn a cross-sectional study, we assessed the presence of LRV1 and LRV2 in 181 clinical isolates of L. major from four endemic cities in Iran using reverse transcription polymerase chain reaction (RT-PCR). After RNA extraction and cDNA synthesis, RT-PCR tests were conducted with LRV1 and LRV2 specific primers. Human beta-actin and kmp genes served as internal and external controls, respectively, and the Allele ID software was used to optimize melting curves.

resultsLRV2 was detected in 27.6% (50 out of 181) of L. major isolates, while no LRV1 was found. We did not observe a statistically significant difference in the presence of LRV2 based on age group, number, or location of lesions.

conclusionThis study confirms the presence of LRV2 in clinical isolates of L. major from endemic regions of Iran. Further researches with larger sample sizes is recommended to explore the association between LRV and clinical symptoms as well as treatment response.

Indexed as

Leishmania majorLeishmaniasis, CutaneousLeishmaniavirusAdolescentAdultChildChild, PreschoolCross-Sectional StudiesEndemic DiseasesFemaleHumansIranMaleMiddle AgedReverse Transcriptase Polymerase Chain ReactionYoung AdultCutaneous LeishmaniasisIranLeishmania MajorLeishmania RNA VirusZCL

Identifiers

PMID39190281

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.