Evidence map›Paper›PMID 39189210›Full record

ArticleBiotech (Basel (Switzerland))2024

Principle Investigation and Method Standardization of Inhibition Zone Assay Based on Antimicrobial Peptides Extracted from Black Soldier Fly Larvae.

Wenyue Shen, Ranxia Xue, Yanxia Liu, Shibo Sun, Xi Chen, Dongye Sun, Han Ouyang, Yuxin Li, Jianqiang Xu, Xiaoying Dong and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Biotech (Basel (Switzerland)), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Wenyue ShenSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Ranxia XueSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Yanxia LiuSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Shibo SunSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Xi ChenInstrumental Analysis and Research Center, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Dongye SunInstrumental Analysis and Research Center, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Han OuyangSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Yuxin LiSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Jianqiang XuSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.ORCID 0000-0002-1289-5943
Xiaoying DongSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Fengyun JiSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.
Weiping XuSchool of Chemical Engineering, Ocean, and Life Sciences & Panjin Institute of Industrial Technology, Dalian University of Technology, Panjin Campus, Panjin 124221, China.ORCID 0000-0002-8904-0206

Funding

Regional Innovation Joint Fund of Liaoning Province 2022-PJLH-03the National Natural Science Foundation of China 52200146Yingkou enterprise and doctor innovation program of Yingkou Science and Technology Bureau 202112
6 · The paper itself

Abstract

The black soldier fly is a valuable resource insect capable of transforming organic waste while producing antimicrobial peptides (AMPs). The inhibition zone assay (IZA) is a method used to demonstrate the antimicrobial activity of AMPs. This study aimed to examine the experimental principles and establish a standardized IZA method. Results indicated that the AMPs extract consisted of proteins ranging in molecular weights from 0 to 40 kDa. The AMPs diffused radially on an agar plate through an Oxford cup. The diffusion radius was influenced by the concentration and volume of the AMPs but ultimately determined by the mass of the AMPs. The swabbing method was found to be effective for inoculating bacteria on the agar plate. Among the conditions tested, the plate nutrient concentration was the most sensitive factor for the IZA, followed by bacterial concentration and incubation time. Optimal conditions for the IZA included a nutrient plate of 0.5× TSA, a bacterial concentration of 10

Indexed as

antimicrobial activityantimicrobial peptidesblack soldier flydiffusioninhibition zone assaystandardization

Identifiers

PMID39189210
PMCPMC11348260

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.