ArticleBMC medical genomics2024
Identification of key immune-related genes and potential therapeutic drugs in diabetic nephropathy based on machine learning algorithms.
Article in BMC medical genomics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.
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3 citing papers in PubMed.
- Transcriptomic Profiling Combined with Machine Learning and Mendelian Randomization Identifies Diagnostic Biomarkers and Immune Infiltration Patterns in Diabetic Kidney Disease.Molecules (Basel, Switzerland) · 2026Article
- Identification of atherosclerosis biomarkers through metabolic signatures and immune microenvironment analysis.Journal of cardiothoracic surgery · 2026Article
- A lipid metabolism-based gene signature defines diagnosis and molecular heterogeneity in diabetic nephropathy.Iranian journal of basic medical sciences · 2026Article
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4 authors.
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Abstract
backgroundDiabetic nephropathy (DN) is a major contributor to chronic kidney disease. This study aims to identify immune biomarkers and potential therapeutic drugs in DN.
methodsWe analyzed two DN microarray datasets (GSE96804 and GSE30528) for differentially expressed genes (DEGs) using the Limma package, overlapping them with immune-related genes from ImmPort and InnateDB. LASSO regression, SVM-RFE, and random forest analysis identified four hub genes (EGF, PLTP, RGS2, PTGDS) as proficient predictors of DN. The model achieved an AUC of 0.995 and was validated on GSE142025. Single-cell RNA data (GSE183276) revealed increased hub gene expression in epithelial cells. CIBERSORT analysis showed differences in immune cell proportions between DN patients and controls, with the hub genes correlating positively with neutrophil infiltration. Molecular docking identified potential drugs: cysteamine, eltrombopag, and DMSO. And qPCR and western blot assays were used to confirm the expressions of the four hub genes.
resultsAnalysis found 95 and 88 distinctively expressed immune genes in the two DN datasets, with 14 consistently differentially expressed immune-related genes. After machine learning algorithms, EGF, PLTP, RGS2, PTGDS were identified as the immune-related hub genes associated with DN. In addition, the mRNA and protein levels of them were obviously elevated in HK-2 cells treated with glucose for 24 h, as well as their mRNA expressions in kidney tissues of mice with DN.
conclusionThis study identified 4 hub immune-related genes (EGF, PLTP, RGS2, PTGDS), as well as their expression profiles and the correlation with immune cell infiltration in DN.
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