Evidence map›Paper›PMID 39187253›Full record

ArticleDiabetes & vascular disease research

Severe hypoglycaemia-induced microglial inflammation damages microvascular endothelial cells, leading to retinal destruction.

Yuxin Hu, Zhen Li, Hongxue Li, Qian Xu, Chengye Xu, Wenjian Lin, Xuefei Ma, Ming Hao, Hongyu Kuang

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Diabetes & vascular disease research. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yuxin HuThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Zhen LiThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Hongxue LiThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Qian XuThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Chengye XuThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Wenjian LinThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Xuefei MaThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Ming HaoThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.
Hongyu KuangThe Department of Endocrinology, The First Affiliated Hospital of Harbin Medical University, Harbin, China.ORCID 0000-0001-6309-9798

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Human microglia (HMC) are stress-induced inflammatory cells of the retina. It is unknown whether severe hypoglycaemia causes inflammation in microglia, affects the permeability of human retinal microvascular endothelial cells (HRMECs), and causes retinal damage. This study aimed to explore the effects of severe hypoglycaemia on retinal microglial inflammation and endothelial cell permeability and evaluate the damage caused by hypoglycaemia to the retina. The CCK-8 assay was used to measure cell viability. Western blotting was used to detect IL-1β, IL-6, TNF- α, claudin-1, and occludin expression. ELISA was used to detect IL-1β, IL-6, and TNF- α. Transmission electron microscopy (TEM) and haematoxylin and eosin staining were used to observe the retinal structure. Immunohistochemistry and immunofluorescence staining assays were also used to detect IL-1β, IL-6, TNF- α, claudin-1, and occludin expression. Severe hypoglycaemia promoted inflammation in HMC3 cells. Inflammation caused by hypoglycaemia leads to the decreased expression of tight junction proteins. In vivo, severe hypoglycaemia induced structural damage to the retina, increased the expression of inflammatory factors, and decreased the expression of tight junction proteins. Our results suggest that severe hypoglycaemia leads to acute retinal inflammation, affecting the permeability of HRMECs and causing retinal damage.

Indexed as

Capillary PermeabilityEndothelial CellsHypoglycemiaInflammation MediatorsMicrogliaRetinal VesselsAnimalsBlood GlucoseBlood-Retinal BarrierCell LineClaudin-1CytokinesDisease Models, AnimalHumansMaleMice, Inbred C57BLBlood GlucoseClaudin-1CLDN1 protein, humanCytokinesInflammation MediatorsOccludinOCLN protein, humanhuman microgliahuman retinal microvascular endothelial cellsinflammationretinal damageSevere hypoglycaemia

Identifiers

PMID39187253
PMCPMC11348349

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.