ArticlePloS one2024
Corynebacterium pseudotuberculosis: Whole genome sequencing reveals unforeseen and relevant genetic diversity in this pathogen.
Article in PloS one, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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6 citing papers in PubMed.
- Up-to-date MALDI-TOF MS-based identification of theJournal of clinical microbiology · 2026Article
- Development and Validation of a High-Resolution Melting (HRM) Method for Differentiating Ovis and Equi Biovars ofVeterinary sciences · 2026Article
- Isolation, whole genome sequencing, and comparative genomics of Corynebacterium pseudotuberculosis to identify biovar ovis specific biomarkers.Molecular biology reports · 2026Article
- Genomic diversity and host-specificity inFrontiers in microbiology · 2026Article
- Comprehensive pathological insights and molecular detection of caseous lymphadenitis caused byJournal of advanced veterinary and animal research · 2026Article
- Diphtheria antitoxin treatment: from pioneer to neglected.Memorias do Instituto Oswaldo Cruz · 2025Review
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Authors and funding
3 authors.
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Abstract
Corynebacterium pseudotuberculosis (CPS) is an important bacterial animal pathogen. CPS causes chronic, debilitating and currently incurable infectious diseases affecting a wide range of livestock and wild herbivores including camelids worldwide. Belonging to the Corynebacterium diphtheriae complex, this pathogen can also infect humans. The classical characterization of CPS is typically based on the testing of nitrate reductase activity, separating the two biovars Equi and Ovis. However, more refined resolutions are required to unravel routes of infection. This was realized in our study by generating and analyzing whole genome sequencing (WGS) data. Using newly created core genome multilocus sequence typing (cgMLST) profiles we were the first to discover isolates grouping in a cluster adjacent to clusters formed by CPS biovar Equi isolates. This novel cluster includes CPS isolates from alpacas, llamas, camels and dromedaries, which are characterized by a lack of nitrate reductase activity as encountered in biovar Ovis. This is of special interest for molecular epidemiology. Nevertheless, these isolates bear the genes of the nitrate locus, which are characteristic of biovar Equi isolates. However, sequence analysis of the genes narG and narH of the nitrate locus revealed indels leading to frameshifts and inactivity of the enzymes involved in nitrate reduction. Interestingly, one CPS isolate originating from another lama with an insertion in the MFS transporter (narT) is adjacent to a cluster formed by ovine CPS isolates biovar Equi. Based on this knowledge, the combination of biochemical and PCR based molecular biological nitrate reductase detection can be used for a fast and uncomplicated classification of isolates in routine diagnostics in order to check the origin of camelid CPS isolates. Further analysis revealed that partial sequencing of the ABC transporter substrate binding protein (CP258_RS07935) is a powerful tool to assign the biovars and the novel genomovar.
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