Evidence map›Paper›PMID 39186142›Full record

ArticlePlant foods for human nutrition (Dordrecht, Netherlands)2024

Inhibitory Effect and Mechanism of Epigallocatechin Gallate on the Differentiation of 3T3-L1 Preadipocytes.

Ranran He, Yu Shi, Xiaoshuang Lu, Yufei Zhou, Zhonghua Liu, Sheng Zhang, Ailing Liu

Abstract read
PubMed Publisher
In one paragraph

Article in Plant foods for human nutrition (Dordrecht, Netherlands), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ranran He *Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China.
Yu Shi *Collaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China.
Xiaoshuang LuCollaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China.
Yufei ZhouCollaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China.
Zhonghua LiuCollaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China.
Sheng ZhangCollaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China. zhangsheng@hunau.edu.cn.
Ailing LiuCollaborative Innovation Centre of Utilisation of Functional Ingredients from Botanicals, National Research Center of Engineering Technology for Utilization of Functional Ingredients from Botanicals, Hunan Agricultural University, Changsha, Hunan, China. liuailing@hunau.edu.cn.

Funding

the Key R&D Project of Hunan Province 2021NK1020-3the National Key R&D Project 2022YFE0111200 & 2021YFD1601104the Natural Science Foundation of Hunan Province Youth Fund No. 2021JJ40251
6 · The paper itself

Abstract

Green tea possesses a range of beneficial effects, including anti-obesity, antioxidant, and anti-inflammatory properties, owing to its biologically active components, primarily catechins such as epicatechin (EC), epicatechin gallate (ECG), epigallocatechin (EGC), and epigallocatechin gallate (EGCG). However, few studies have investigated the four catechin monomers simultaneously, and the molecular mechanisms of their anti-obesity effects have not been fully elucidated. In this study, we investigated the effects of four catechin monomers on the differentiation of 3T3-L1 preadipocytes of mice. Our findings demonstrated that four catechin monomers EC/ECG/EGC/EGCG (12, 25, 50 µM) dose-dependently inhibited the differentiation of 3T3-L1 preadipocytes and reduced triglyceride content. EGCG exhibited the most potent inhibitory effect with an optimal concentration of 50 µM. In addition, transcriptome sequencing and lipidomic analysis of EGCG-treated 3T3-L1 preadipocytes revealed that Ptgs2 and Pim1 were the most differentially expressed genes involved in regulating adipocyte differentiation. The results suggested that EGCG up-regulated the expression of the Pla2g2e gene and down-regulated the expression of the Pla2g4a and Pla2g2a genes via the glycerophospholipid metabolic pathway, which subsequently elevated lysophosphatidylcholine (LPC) levels, influencing the differentiation process of 3T3-L1 preadipocytes.

Indexed as

3T3-L1 CellsAdipocytesCatechinCell DifferentiationTriglyceridesAdipogenesisAnimalsAnti-Obesity AgentsCyclooxygenase 2MiceTeaAnti-Obesity AgentsCatechinCyclooxygenase 2epicatechin gallateepigallocatechin gallategallocatecholPtgs2 protein, mouseTeaTriglycerides3T3-L1 preadipocytesEpigallocatechin gallateLipidomicsObesityTranscriptomics

Identifiers

PMID39186142

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.