ArticleThe Journal of cell biology2024
Near millimolar concentration of nucleosomes in mitotic chromosomes from late prometaphase into anaphase.
Article in The Journal of cell biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Chromosome crowding shapes spindle geometry.Nature cell biology · 2026Article
- Power-law scaling of mitotic spindles with genome sizes across eukaryotes is driven by chromosome crowding.Nature cell biology · 2026Article
- Spindle pole proteins confine chromosomes to ensure their expulsion during female meiosis.bioRxiv : the preprint server for biology · 2026Article
- Uncovering mitotic ultrastructure in the native hair follicle using volume electron microscopy.Journal of cell science · 2026Article
- Dynamics of microcompartment formation at the mitosis-to-G1 transition.Nature structural & molecular biology · 2025Article
- Mitotic genome folding.The Journal of cell biology · 2025Review
- Nanoscale analysis of human G1 and metaphase chromatin in situ.The EMBO journal · 2025Article
- How condensed are mitotic chromosomes?The Journal of cell biology · 2024Article
Corrections and comments
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Authors and funding
9 authors.
Funding
Abstract
Chromosome compaction is a key feature of mitosis and critical for accurate chromosome segregation. However, a precise quantitative analysis of chromosome geometry during mitotic progression is lacking. Here, we use volume electron microscopy to map, with nanometer precision, chromosomes from prometaphase through telophase in human RPE1 cells. During prometaphase, chromosomes acquire a smoother surface, their arms shorten, and the primary centromeric constriction is formed. The chromatin is progressively compacted, ultimately reaching a remarkable nucleosome concentration of over 750 µM in late prometaphase that remains relatively constant during metaphase and early anaphase. Surprisingly, chromosomes then increase their volume in late anaphase prior to deposition of the nuclear envelope. The plateau of total chromosome volume from late prometaphase through early anaphase described here is consistent with proposals that the final stages of chromatin condensation in mitosis involve a limit density, such as might be expected for a process involving phase separation.
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Registered trials
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