Evidence map›Paper›PMID 39185998›Full record

ArticleAnimal biotechnology2024

Spatial distribution of maternal factors in pig mature oocytes.

Fuquan Zhu, Meng Yang, Dayu Wang, Yuan Jiang, Chao Jia, Yanfeng Fu, Aochen Yu, Huijun Liu, Meixia Wang, Tingzhang Wang and 2 more

Abstract read
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In one paragraph

Article in Animal biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

12 authors.

Fuquan ZhuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Meng YangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Dayu WangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Yuan JiangCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Chao JiaCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Yanfeng FuInstitute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing, China.
Aochen YuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Huijun LiuKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, Zhejiang Province, China.
Meixia WangKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, Zhejiang Province, China.
Tingzhang WangKey Laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, Zhejiang Institute of Microbiology, Hangzhou, Zhejiang Province, China.
Honglin LiuCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.
Juan LiCollege of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

It is known that asymmetrical maternal transcripts play an important role in the cell fate of the early embryo, but few studies are available in mammal oocytes especially in pig. To investigate the spatial factors in pig oocytes, the oriented bisection was established for collecting karyoplasts (NSOs) and cytoplasts (SSOs) with more than 95% efficiency. Subsequently, RNA-Seq and LC-MS/MS analysis were performed on NSOs and SSOs. Although no differentially expressed genes (DEGs) could be detected between NSOs and SSOs, 89 of the differentially expressed proteins (DEPs) were detected, that 58 proteins higher expressed but 31 proteins lower expressed in NSOs compared with SSOs. These DEPs mainly participated in the 'cell cycle' and 'ribosome' pathway, while the up-regulated DEPs were mainly GO in 'spindle' and 'positive regulation of translation', and the down-regulated DEPs were in 'cytosolic small ribosomal subunit' and 'mRNA binding'. The up-regulated DEP SIRT5 which are related to the regulation of gene expression, epigenetic were further detected and revealed. A spatial asymmetry of maternal factors at the protein level was firstly detected in pig mature oocytes.

Indexed as

OocytesAnimalsFemaleSwinematernal factorsoriented bisectionPorcineproteomicstranscriptomics

Identifiers

PMID39185998
PMCPMC12674313

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.