Evidence map›Paper›PMID 39185225›Full record

ArticlebioRxiv : the preprint server for biology2024

Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.

Mighten C Yip, Mercedes M Gonzalez, Colby F Lewallen, Corey R Landry, Ilya Kolb, Bo Yang, William M Stoy, Ming-Fai Fong, Matthew J M Rowan, Edward S Boyden and 1 more

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

11 authors.

Mighten C YipGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.ORCID 0000-0002-8463-0311
Mercedes M GonzalezGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.
Colby F LewallenOcular and Stem Cell Translational Research Section, Ophthalmic Genetics and Visual Function Branch, National Eye Institute, National Institutes of Health, Bethesda, MD, 20892, USA.
Corey R LandryWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.
Ilya KolbGENIE Project Team, Janelia Research Campus Howard Hughes Medical Institute, Ashburn, VA, 20147, USA.
Bo YangGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.
William M StoyDepartment of Electrical Engineering, Columbia University, New York, NY, 10027, USA.ORCID 0000-0001-9425-0809
Ming-Fai FongWallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.ORCID 0000-0002-2336-4531
Matthew J M RowanDepartment of Cell Biology, Emory University, 615 Michael St, Atlanta, GA, 30322, USA.ORCID 0000-0003-0955-0706
Edward S BoydenDepartment of Brain and Cognitive Science, Massachusetts Institute of Technology, Cambridge, MA, USA.ORCID 0000-0002-0419-3351
Craig R ForestGeorge W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, 315 Ferst Dr, Atlanta, GA, 30363, USA.

Funding

Novel Platforms for Systematic Optical Control of Complex Neural Circuits In VivoR01DA029639 · NIDA · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S. · 2010 to 2023
$6.7M
High-throughput robotic analysis of integrated neuronal phenotypesR01EY023173 · NEI · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2012 to 2016
$4.3M
Scalable Cell- and Circuit-Targeted ElectrophysiologyR01NS102727 · NINDS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI BOYDEN, EDWARD S., FOREST, CRAIG · 2017 to 2020
$2.2M
In-vivo circuit activity measurement at single cell, sub-threshold resolutionU01MH106027 · NIMH · GEORGIA INSTITUTE OF TECHNOLOGY · PI FOREST, CRAIG, STANLEY, GARRETT B. · 2014 to 2016
$1.5M
NEI NIH HHS R01 EY023173NIDA NIH HHS R01 DA029639NIMH NIH HHS U01 MH106027NINDS NIH HHS R01 NS102727
6 · The paper itself

Abstract

Significant technical challenges exist when measuring synaptic connections between neurons in living brain tissue. The patch clamping technique, when used to probe for synaptic connections, is manually laborious and time-consuming. To improve its efficiency, we pursued another approach: instead of retracting all patch clamping electrodes after each recording attempt, we cleaned just one of them and reused it to obtain another recording while maintaining the others. With one new patch clamp recording attempt, many new connections can be probed. By placing one pipette in front of the others in this way, one can "walk" across the tissue, termed "patch-walking." We performed 136 patch clamp attempts for two pipettes, achieving 71 successful whole cell recordings (52.2%). Of these, we probed 29 pairs (i.e., 58 bidirectional probed connections) averaging 91

Indexed as

automationpaired recordingpatch clampsynaptic connectivity

Identifiers

PMID39185225
PMCPMC11343158

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.