Evidence map›Paper›PMID 39185217›Full record

ArticlebioRxiv : the preprint server for biology2024

Chromatin remodeler CHD4 establishes chromatin states required for ovarian reserve formation, maintenance, and germ cell survival.

Yasuhisa Munakata, Mengwen Hu, Yuka Kitamura, Adam L Bynder, Amelia S Fritz, Richard M Schultz, Satoshi H Namekawa

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Yasuhisa MunakataDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Mengwen HuDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Yuka KitamuraDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Adam L BynderDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Amelia S FritzDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Richard M SchultzDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.
Satoshi H NamekawaDepartment of Microbiology and Molecular Genetics, University of California, Davis, CA 95616, USA.ORCID 0000-0002-1052-943X

Funding

Epigenetic gene regulations in the germlineR35GM141085 · NIGMS · UNIVERSITY OF CALIFORNIA AT DAVIS · PI Satoshi Namekawa · 2021 to 2026
$4.4M
Ovarian reserve formation and maintenanceR21HD110146 · NICHD · UNIVERSITY OF CALIFORNIA AT DAVIS · PI NAMEKAWA, SATOSHI, SCHULTZ, RICHARD MORRIS · 2023 to 2024
$440k
NICHD NIH HHS R21 HD110146NIGMS NIH HHS R35 GM141085
6 · The paper itself

Abstract

The ovarian reserve defines female reproductive lifespan, which in humans spans decades due to the maintenance of meiotic arrest in non-growing oocytes (NGO) residing in primordial follicles. Unknown is how the chromatin state of NGOs is established to enable long-term maintenance of the ovarian reserve. Here, we show that a chromatin remodeler, CHD4, a member of the Nucleosome Remodeling and Deacetylase (NuRD) complex, establishes chromatin states required for formation and maintenance of the ovarian reserve. Conditional loss of CHD4 in perinatal mouse oocytes results in acute death of NGOs and depletion of the ovarian reserve. CHD4 establishes closed chromatin at regulatory elements of pro-apoptotic genes to prevent cell death and at specific genes required for meiotic prophase I to facilitate the transition from meiotic prophase I oocytes to meiotic arrested NGOs. In addition, CHD4 establishes closed chromatin at the regulatory elements of pro-apoptotic genes in male germ cells, allowing male germ cell survival. These results demonstrate a role for CHD4 in defining a chromatin state that ensures germ cell survival, thereby enabling the long-term maintenance of both female and male germ cells.

Identifiers

PMID39185217
PMCPMC11343143

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.