Evidence map›Paper›PMID 39185166›Full record

ArticlebioRxiv : the preprint server for biology2024

Sequence-Directed Covalent Protein-RNA Linkages in a Single Step Using Engineered HUH-Tags.

Adam T Smiley, Natalia Babilonia-Díaz, August J Krueger, Hideki Aihara, Kassidy J Tompkins, Andrew C D Lemmex, Wendy R Gordon

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

7 authors.

Adam T SmileyUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.ORCID 0000-0002-3891-8242
Natalia Babilonia-DíazUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.
August J KruegerUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.
Hideki AiharaUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.
Kassidy J TompkinsUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.
Andrew C D LemmexUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.
Wendy R GordonUniversity of Minnesota, Department of Biochemistry, Molecular Biology, and Biophysics.ORCID 0000-0001-7696-5560

Funding

TRAINING PROGRAM IN MUSCLE RESEARCHT32AR007612 · NIAMS · UNIVERSITY OF MINNESOTA TWIN CITIES · PI JAMES M ERVASTI, DAWN A LOWE · 2001 to 2026
$11.3M
Structural studies of viral replication and invasionR35GM118047 · NIGMS · UNIVERSITY OF MINNESOTA · PI Hideki Aihara · 2016 to 2026
$6.3M
Mechanism and modulation of Amyloid Precursor Protein proteolysisR35GM119483 · NIGMS · UNIVERSITY OF MINNESOTA · PI WENDY RYAN GORDON · 2016 to 2026
$4.6M
Training the Next Generation of Chemical BiologistsT32GM132029 · NIGMS · UNIVERSITY OF MINNESOTA · PI Erin Elizabeth Carlson, William Charles Krause Pomerantz · 2019 to 2026
$2.7M
NIAMS NIH HHS T32 AR007612NIGMS NIH HHS R35 GM118047NIGMS NIH HHS R35 GM119483NIGMS NIH HHS T32 GM132029
6 · The paper itself

Abstract

Replication-initiating HUH-endonucleases (Reps) are enzymes that form covalent bonds with single-stranded DNA (ssDNA) in a sequence specific manner to initiate rolling circle replication. These nucleases have been co-opted for use in biotechnology as sequence specific protein-ssDNA bioconjugation fusion partners dubbed 'HUH-tags'. Here, we describe the engineering and

Identifiers

PMID39185166
PMCPMC11343116

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.