Evidence map›Paper›PMID 39184441›Full record

ArticleiScience2024

Long-working-distance high-collection-efficiency three-photon microscopy for

Peng Deng, Shoupei Liu, Yaoguang Zhao, Xinxin Zhang, Yufei Kong, Linlin Liu, Yujie Xiao, Shasha Yang, Jiahao Hu, Jixiong Su and 10 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

20 authors.

Peng DengDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Shoupei LiuDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Yaoguang ZhaoDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Xinxin ZhangInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China.
Yufei KongInstitute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
Linlin LiuInstitute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
Yujie XiaoDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Shasha YangDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200032, China.
Jiahao HuDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Jixiong SuDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Ang XuanDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Jinhong XuInstitute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
Huijuan LiInstitute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
Xiaoman SuState Key Laboratory of Medical Neurobiology, Institutes of Brain Science, MOE Frontiers Center for Brain Science, Department of Neurology of Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Jingchuan WuDepartment of Neurosurgery, Huashan Hospital, Fudan University, Shanghai 200032, China.
Yuli JiangDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Yu MuInstitute of Neuroscience, State Key Laboratory of Neuroscience, Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai 200031, China.
Zhicheng ShaoInstitute for Translational Brain Research, State Key Laboratory of Medical Neurobiology, MOE Frontiers Center for Brain Science, Institute of Pediatrics, National Children's Medical Center, Children's Hospital, Fudan University, Shanghai 200032, China.
Cihang KongDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.
Bo LiDepartment of Neurosurgery, Huashan Hospital, MOE Frontiers Center for Brain Science, State Key Laboratory of Medical Neurobiology, Institutes for Translational Brain Research, Fudan University, Shanghai 200032, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Zebrafish and organoids, crucial for complex biological studies, necessitate an imaging system with deep tissue penetration, sample protection from environmental interference, and ample operational space. Traditional three-photon microscopy is constrained by short-working-distance objectives and falls short. Our long-working-distance high-collection-efficiency three-photon microscopy (LH-3PM) addresses these challenges, achieving a 58% fluorescence collection efficiency at a 20 mm working distance. LH-3PM significantly outperforms existing three-photon systems equipped with the same long working distance objective, enhancing fluorescence collection and dramatically reducing phototoxicity and photobleaching. These improvements facilitate accurate capture of neuronal activity and an enhanced detection of activity spikes, which are vital for comprehensive, long-term imaging. LH-3PM's imaging of epileptic zebrafish not only showed sustained neuron activity over an hour but also highlighted increased neural synchronization and spike numbers, marking a notable shift in neural coding mechanisms. This breakthrough paves the way for new explorations of biological phenomena in small model organisms.

Indexed as

Biological sciencesBiological sciences research methodologiesOptical imaging

Identifiers

PMID39184441
PMCPMC11342284

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.