Evidence map›Paper›PMID 39183326›Full record

ArticleCommunications biology2024

Mouse guanylate-binding proteins of the chromosome 3 cluster do not mediate antiviral activity in vitro or in mouse models of infection.

Melkamu B Tessema, Shouya Feng, Daniel Enosi Tuipulotu, Rubaiyea Farrukee, Chinh Ngo, Catarina Gago da Graça, Masahiro Yamomoto, Daniel T Utzschneider, Andrew G Brooks, Sarah L Londrigan and 2 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Communications biology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Melkamu B TessemaDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.ORCID 0000-0003-3619-9800
Shouya FengDivision of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0002-3529-3822
Daniel Enosi TuipulotuDivision of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0002-6442-4633
Rubaiyea FarrukeeDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.
Chinh NgoDivision of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.
Catarina Gago da GraçaDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.
Masahiro YamomotoDepartment of Immunoparasitology, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan.ORCID 0000-0002-6821-2785
Daniel T UtzschneiderDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.ORCID 0000-0003-2205-9057
Andrew G BrooksDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.ORCID 0000-0002-4085-9683
Sarah L LondriganDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia.
Si Ming ManDivision of Immunology and Infectious Disease, The John Curtin School of Medical Research, The Australian National University, Canberra, Australia.ORCID 0000-0002-5079-2857
Patrick C ReadingDepartment of Microbiology and Immunology, University of Melbourne, at The Peter Doherty Institute for Infection and Immunity, 792 Elizabeth St, Victoria, 3000, Australia. preading@unimelb.edu.au.ORCID 0000-0002-8860-5308

Funding

Department of Health | National Health and Medical Research Council (NHMRC) APP1143154
6 · The paper itself

Abstract

Dynamin-like GTPase proteins, including myxoma (Mx) and guanylate-binding proteins (GBPs), are among the many interferon stimulated genes induced following viral infections. While studies report that human (h)GBPs inhibit different viruses in vitro, few have convincingly demonstrated that mouse (m)GBPs mediate antiviral activity, although mGBP-deficient mice have been used extensively to define their importance in immunity to diverse intracellular bacteria and protozoa. Herein, we demonstrate that individual (overexpression) or collective (knockout (KO) mice) mGBPs of the chromosome 3 cluster (mGBPchr3) do not inhibit replication of five viruses from different virus families in vitro, nor do we observe differences in virus titres recovered from wild type versus mGBPchr3 KO mice after infection with three of these viruses (influenza A virus, herpes simplex virus type 1 or lymphocytic choriomeningitis virus). These data indicate that mGBPchr3 do not appear to be a major component of cell-intrinsic antiviral immunity against the diverse viruses tested in our studies.

Indexed as

GTP-Binding ProteinsMice, KnockoutAnimalsDisease Models, AnimalHerpesvirus 1, HumanLymphocytic choriomeningitis virusMiceMice, Inbred C57BLVirus DiseasesVirus ReplicationGTP-Binding Proteins

Identifiers

PMID39183326
PMCPMC11345437

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.