Evidence map›Paper›PMID 39181252›Full record

ArticleThe Science of the total environment2024

Quantitative fecal pollution assessment with bacterial, viral, and molecular methods in small stream tributaries.

Brian R McMinn, Asja Korajkic, Julie Kelleher, Adam Diedrich, Adin Pemberton, Jessica R Willis, Mano Sivaganesan, Brooke Shireman, Andrew Doyle, Orin C Shanks

Abstract read
In one paragraph

Article in The Science of the total environment, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Brian R McMinnCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States. Electronic address: mcminn.brian@epa.gov.
Asja KorajkicCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Julie KelleherCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Adam DiedrichCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Adin PembertonCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Jessica R WillisCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Mano SivaganesanCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.
Brooke ShiremanSanitation District No. 1 of Northern Kentucky, 1045 Eaton Drive, Fort Wright, KY 41017, United States.
Andrew DoyleSanitation District No. 1 of Northern Kentucky, 1045 Eaton Drive, Fort Wright, KY 41017, United States.
Orin C ShanksCenter for Environmental Measurement and Modeling, Office of Research and Development, United States Environmental Protection Laboratory, 26 West Martin Luther King Drive, Cincinnati, OH 45268, United States.

Funding

Intramural EPA EPA999999
6 · The paper itself

Abstract

Stream water quality can be impacted by a myriad of fecal pollution sources and waste management practices. Identifying origins of fecal contamination can be challenging, especially in high order streams where water samples are influenced by pollution from large drainage areas. Strategic monitoring of tributaries can be an effective strategy to identify conditions that influence local water quality. Water quality is assessed using fecal indicator bacteria (FIB); however, FIB cannot differentiate sources of fecal contamination nor indicate the presence of disease-causing viruses. Under different land use scenarios, three small stream catchments were investigated under 'wet' and 'dry' conditions (Scenario 1: heavy residential; Scenario 2: rural residential; and Scenario 3: undeveloped/agricultural). To identify fecal pollution trends, host-associated genetic targets HF183/BacR287 (human), Rum2Bac (ruminant), GFD (avian), and DG3 (canine) were analyzed along with FIB (Escherichia coli and enterococci), viral indicators (somatic and F+ coliphage), six general water quality parameters, and local rainfall. Levels of E. coli exceeded single sample maximum limits (235 CFU/100 mL) in 70.7 % of samples, enterococci (70 CFU/100 mL) in 100 % of samples, and somatic coliphage exceeded advisory thresholds (600 PFU/L) in 34.1 % of samples. The detection frequency for the human-associated genetic marker was highest in Scenario 3 (50 % of samples) followed by Scenario 2 (46 %), while the ruminant-associated marker was most prevalent in Scenario 1 (64 %). Due to the high proportion of qPCR-based measurements below the limit of quantification, a Bayesian data analysis approach was applied to investigate links between host-associated genetic marker occurrence with that of rainfall and fecal indicator levels. Multiple trends associated with small stream monitoring were revealed, emphasizing the role of rainfall, the utility of fecal source information to improve water quality management. And furthermore, water quality monitoring with bacterial or viral methodologies can alter the interpretation of fecal pollution sources in impaired waters.

Indexed as

Environmental MonitoringFecesRiversWater MicrobiologyWater PollutionWater QualityEscherichia coliColiphageFecal indicator bacteriaMicrobial source trackingStream tributaryWater quality

Identifiers

PMID39181252
PMCPMC11462285

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.