Evidence map›Paper›PMID 39180671›Full record

ArticleMolecular biology reports2024

Construction of pVAX-1-based linear covalently closed vector with improved transgene expression.

Kevin Kumar Vijayakumar, Devaprakash Manoharan, Rajasekaran Subbarayan, Rupendra Shrestha, Shakila Harshavardhan

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Article in Molecular biology reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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3 · Its place in the literature

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4 · The record

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5 · Who and what money

Authors and funding

5 authors.

Kevin Kumar VijayakumarDepartment of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu, 625021, India.ORCID http://orcid.org/0000-0002-8761-3242
Devaprakash ManoharanDepartment of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu, 625021, India.ORCID http://orcid.org/0009-0000-5963-9763
Rajasekaran SubbarayanCentre for Advanced Biotherapeutic and Regenerative Medicine, Faculty of Research, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, India.ORCID http://orcid.org/0000-0001-5890-5273
Rupendra ShresthaResearch and Collaboration, Anka Analytica, Melbourne, Australia.ORCID http://orcid.org/0000-0001-6804-6070
Shakila HarshavardhanDepartment of Molecular Microbiology, School of Biotechnology, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu, 625021, India. mohanshakila.biotech@mkuniversity.ac.in.ORCID http://orcid.org/0000-0002-9093-5621

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

introductionThis study presents a Mammalian Linear Expression System (MLES), a linear covalently closed (LCC) vector based on pVAX-1. The purpose of this system was to improve gene expression in mammalian cells and to test the efficacy of MLES in transient transfection and transgene expression using in vitro and in vivo models. Additionally, we aimed to evaluate potential inflammatory responses in vivo. MATERIALS AND

methodsMLES was developed by modifying pVAX-1, and the construct was confirmed by gel electrophoresis. Lipofectamine

resultsMLES exhibited higher transfection efficiency and expression levels compared to pVAX-1 when tested on HEK-293, CHO-K1, and NIH-3T3 cells. When tested in vivo, MLES/EGFP showed elevated expression in the heart, kidney, liver, and spleen compared with pVAX-1/EGFP. Minimal changes are observed in the lungs. Additionally, MLES induced a reduced inflammatory response in mice compared with pVAX-1/EGFP.

conclusionsMLES offer improved transfection efficiency and reduced inflammation, representing a significant advancement in gene therapy and recombinant protein production. Further research on MLES-mediated gene expression and immune modulation will enhance gene therapy strategies.

Indexed as

CricetulusGene ExpressionGenetic VectorsTransfectionTransgenesAnimalsCHO CellsGreen Fluorescent ProteinsHEK293 CellsHumansMiceNIH 3T3 Cellsenhanced green fluorescent proteinGreen Fluorescent ProteinsGene expressionGene therapyInflammationLinear covalently closed vectorMammalian linear expression systemTransient transfection

Identifiers

PMID39180671

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.