Evidence map›Paper›PMID 39180641›Full record

ArticleJournal of neuro-oncology2024

Pulsed focused ultrasound alters the proteomic profile of the tumor microenvironment in a syngeneic mouse model of glioblastoma.

Hui Chen, Dimpy Koul, Yanrong Zhang, Sara Natasha Ghobadi, Yayu Zhu, Qingyi Hou, Edwin Chang, Frezghi G Habte, Ramasamy Paulmurugan, Sabbir Khan and 5 more

Abstract read
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In one paragraph

Article in Journal of neuro-oncology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

15 authors.

Hui ChenDepartment of Neuroradiology, The University of Texas MD Anderson Cancer Center, 1400 Pressler St, Unit 1482, Houston, TX, 77030, USA.
Dimpy KoulDepartment of Neuroradiology, The University of Texas MD Anderson Cancer Center, 1400 Pressler St, Unit 1482, Houston, TX, 77030, USA.
Yanrong ZhangDepartment of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA.
Sara Natasha GhobadiDepartment of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA.
Yayu ZhuSalpointe Catholic High School, Tucson, AZ, USA.
Qingyi HouDepartment of Radiology, Neuroradiology Division, Stanford University, Stanford, CA, USA.
Edwin ChangDepartment of Radiology, Molecular Imaging Program, Stanford University, Stanford, CA, USA.
Frezghi G HabteDepartment of Radiology, Molecular Imaging Program, Stanford University, Stanford, CA, USA.
Ramasamy PaulmuruganDepartment of Radiology, Molecular Imaging Program, Stanford University, Stanford, CA, USA.
Sabbir KhanDepartment of Neuro-Oncology, The University of Texas MD Anderson Cancer Center, Houston, TX, USA.
Yuqi ZhengKen Parker Brain Tumour Research Laboratories, Brain and Mind Centre, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, 2050, Australia.
Manuel B GraeberKen Parker Brain Tumour Research Laboratories, Brain and Mind Centre, Faculty of Medicine and Health, University of Sydney, Camperdown, NSW, 2050, Australia.
Iris HerschmannThe Human Immune Monitoring Center (HIMC), Stanford University, Stanford, CA, USA.
Kevin S LeeDepartments of Neuroscience and Neurosurgery, Center for Brain Immunology and Glia, School of Medicine, University of Virginia, 409 Lane Road, MR4 Building, PO Box 801392, Charlottesville, VA, 22903, USA. ksl3h@virginia.edu.
Max WintermarkDepartment of Neuroradiology, The University of Texas MD Anderson Cancer Center, 1400 Pressler St, Unit 1482, Houston, TX, 77030, USA. max.wintermark@gmail.com.

Funding

Translational Oncology Research Program (Project-005)P30CA124435 · NCI · STANFORD UNIVERSITY · PI MICHAEL KENNEY · 2007 to 2026
$71.4M
MR-Guided Focused Ultrasound Combined with Immunotherapy to Treat Malignant Brain TumorsR01CA217953 · NCI · STANFORD UNIVERSITY · PI HEIT, JEREMY J · 2017 to 2021
$3.1M
National Institutes of Health Grant R01 CA217953-01NCI NIH HHS P30 CA124435NCI NIH HHS R01 CA217953
6 · The paper itself

Abstract

purposeGlioblastoma (GBM), a lethal primary adult malignancy, is difficult to treat because of the restrictive nature of the blood-brain barrier (BBB), blood-tumor barrier (BTB), and the immunosuppressive tumor microenvironment (TME). Since pulsed focused ultrasound (pFUS) is currently used to improve therapeutic deliveries across these barriers, this study aims to characterize the impact of pFUS on the TME proteomics upon opening the BBB and BTB.

methodsWe utilized MRI-guided, pFUS with ultrasound contrast microbubbles (termed 'pFUS' herein) to selectively and transiently open the BBB and BTB investigating proteomic modifications in the TME. Utilizing an orthotopically-allografted mouse GL26 GBM model (Ccr2RFP/wt - Cx3cr1GFP/wt), pFUS's effect on glioma proteomics was evaluated using a Luminex 48-plex assay.

resultspFUS treated tumors exhibited increases in pro-inflammatory cytokines, chemokines, and trophic factors (CCTFs). Proteomic changes in tumors tend to peak at 24 h after single pFUS session (1x), with levels then plateauing or declining over the subsequent 24 h. Tumors receiving three pFUS sessions (3x) showed elevated CCTFs levels peaking as early as 6 h after the third session.

conclusionspFUS together with microbubbles induces a sterile inflammatory response in the TME of a mouse GBM tumor. Moreover, this proinflammatory shift can be sustained and perhaps primed for more rapid responses upon multiple sessions of pFUS. These findings raise the intriguing potential that pFUS-induced BBB and BTB opening may not only be effective in facilitating the therapeutic agent delivery, but also be harnessed to modify the TME to assist immunotherapies in overcoming immune evasion in GBM.

Indexed as

Blood-Brain BarrierBrain NeoplasmsDisease Models, AnimalGlioblastomaProteomicsTumor MicroenvironmentAnimalsMiceMice, Inbred C57BLMicrobubblesUltrasonic TherapyGlioblastoma mouse modelImmunomodulationProteomic changesPulsed Focused UltrasoundTumor microenvironment

Identifiers

PMID39180641
PMCPMC12313442

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.