Evidence map›Paper›PMID 39180435›Full record

ArticleThe Laryngoscope2025

Establishing a Mouse Model of Surgical Vocal Fold Injury.

Akari Kimura, Mohammed Imran Khan, Meena Easwaran, Joanne Soo, Amirbahador Golchin, Elizabeth Erickson-DiRenzo

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Laryngoscope, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Akari KimuraDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.ORCID 0000-0001-6682-8212
Mohammed Imran KhanDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.
Meena EaswaranDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.
Joanne SooDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.
Amirbahador GolchinDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.
Elizabeth Erickson-DiRenzoDepartment of Otolaryngology - Head & Neck surgery, School of Medicine, Stanford University, Stanford, California, USA.ORCID 0000-0002-9841-0184

Funding

Evaluating the role of epithelial basal cells in laryngeal homeostasis and disease developmentR01DC020176 · NIDCD · STANFORD UNIVERSITY · PI Elizabeth DiRenzo · 2022 to 2026
$1.9M
NIDCD NIH HHS R01 DC020176NIH HHS
6 · The paper itself

Abstract

objectiveAnimal models of vocal fold (VF) surgical injury and scar formation provide insight into the wound healing process. The purpose of this study was to establish an alternative model of surgical injury to the mouse VF using materials commonly available in most research laboratories or for purchase and to investigate wound healing of the epithelium (EP) and lamina propria (LP).

methodsMice were anesthetized by isoflurane gas delivery and positioned on a platform so that the larynx could be observed using a laryngoscope and dissection microscope. Unilateral VF injury was created using a wire brush. Mice were euthanized and the larynx evaluated 1-, 3-, 5-, 7-, 14-, and 28-days following injury. Histological and immunofluorescent analysis was used to evaluate thickness of the EP, LP area, proliferative (Ki67+) and basal cells (p63+) in the EP, and collagen III content in the LP.

resultsThe depth of injury reached the superficial thyroarytenoid muscle on Day 1. The thickness of the EP of the injured VF was increased on Days 3 and 5, and the LP area was increased on Days 3, 5, and 7 as compared with the uninjured VF. Ki67+ and p63+ cells were increased on Day 3 and collagen III content was increased on Days 5 and 28 as compared with the uninjured VF.

conclusionWe successfully established an alternative method of creating unilateral VF injury in the mouse. This method will be useful for future research regarding VF surgical injury and wound healing. LEVEL OF EVIDENCE: NA Laryngoscope, 135:213-222, 2025.

Indexed as

Disease Models, AnimalVocal CordsWound HealingAnimalsCicatrixMaleMiceMice, Inbred C57BLMucous Membranemouse modelvocal fold mucosavocal fold surgical injurywound healing

Identifiers

PMID39180435
PMCPMC11637976

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.