Evidence map›Paper›PMID 39179691›Full record

ReviewThe protein journal2024

Characterization of the Three DHFRs and K65P Variant: Enhanced Substrate Affinity and Molecular Dynamics Analysis.

Ruirui Feng, Shuanghao Yang, Xingchu Zhao, Bo Sun, Shengkai Zhang, Qirong Shen, Qun Wan

Abstract readReview
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In one paragraph

Review in The protein journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ruirui FengCollege of Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Shuanghao YangCollege of Science, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Xingchu ZhaoKey Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Bo SunKey Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Shengkai ZhangInstitute of Advanced Science Facilities, Shenzhen, 518107, People's Republic of China.
Qirong ShenKey Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China.
Qun WanKey Lab of Organic-Based Fertilizers of China and Jiangsu Provincial Key Lab for Solid Organic Waste Utilization, Nanjing Agricultural University, Nanjing, 210095, People's Republic of China. qunwan@njau.edu.cn.

Funding

Nanjing Agricultural University XUEKEN2023039the National Natural Science Foundation of China 12005248the National Natural Science Foundation of China 32071264
6 · The paper itself

Abstract

Dihydrofolate reductase (DHFR) is ubiquitously present in all living organisms and plays a crucial role in the growth of the fungal pathogen R.solani. Sequence alignment confirmed the evolutionary conservation of the essential lid domain, with the amino acid 'P' within the PEKN lid domain appearing with a frequency of 89.5% in higher organisms and 11.8% in lower organisms. Consequently, a K65P variant was introduced into R.solani DHFR (rDHFR). Subsequent enzymatic kinetics assays were conducted for human DHFR (hDHFR), rDHFR, E. coli DHFR (eDHFR), and the K65P variant. hDHFR exhibited the highest k

Indexed as

Molecular Dynamics SimulationTetrahydrofolate DehydrogenaseAmino Acid SubstitutionEscherichia coliFolic AcidFungal ProteinsHumansKineticsNADPSubstrate SpecificityFolic AcidFungal ProteinsNADPTetrahydrofolate DehydrogenaseDihydrofolate reductaseEnzyme kineticsProtein purificationRecombinant protein expression

Identifiers

PMID39179691

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.