Evidence map›Paper›PMID 39179321›Full record

ArticleJournal, genetic engineering & biotechnology2024

Whole genome sequence analysis of Bacillus amyloliquefaciens strain S2.5 as a potential probiotic for feed supplement in livestock production.

Ha-Anh Nguyen, Thao Tran P, Hang Thuy Dam, Hai Van Nguyen, Thanh Ha Le, Phu-Ha Ho, Nguyen Lan Huong

Abstract read
In one paragraph

Article in Journal, genetic engineering & biotechnology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.

0numbers the graph read from it
0cells of the map it votes in
11citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

11 citing papers in PubMed.

  1. Article
  2. Small non-coding RNAMicrobiology spectrum · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Multifunctional Bioactivity ofCurrent issues in molecular biology · 2026
    Article
  7. Article
  8. DietaryFrontiers in veterinary science · 2026
    Article
  9. Article
  10. Article
  11. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Ha-Anh NguyenSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam.
Thao Tran PSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam.
Hang Thuy DamSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam.
Hai Van NguyenSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam.
Thanh Ha LeSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam.
Phu-Ha HoSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam. Electronic address: ha.hophu@hust.edu.vn.
Nguyen Lan HuongSchool of Chemistry and Life Sciences (former School of Biotechnology and Food Technology), Hanoi University of Science and Technology, Hanoi, Viet Nam. Electronic address: huong.nguyenlan@hust.edu.vn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundSupplementing probiotics in livestock feed is increasing due to concerns over the potential harm caused by antibiotics and other chemical growth promoters. Several Bacillus sp. have been used as probiotic supplements for livestock. In this study, Bacillus amyloliquefaciens S2.5 was isolated from freshwater and its potential probiotic characteristics were evaluated in vitro. The whole genome of strain S2.5 was sequenced, and its probiotic traits were annotated using bioinformatic tools.

resultsBoth vegetative cells and spores of strain S2.5 remained stable throughout the 1.5 h of gastric juice and 48 h of intestine simulation. The strain S2.5 harbored the ability to produce glucoamylase, carboxymethyl cellulase, protease, and chitinase. It is also susceptible to all six tested antibiotics. The complete genome sequence shows genes related to acid-bile tolerance, environmental stress resistance, hydrolases, and adhesion to gut mucosa, confirming probiotic traits in the in vitro experiments.

conclusionsB. amyloliquefaciens S2.5 demonstrated potential probiotic characteristics and its genetic profile in the in vitro experiments. Further in vivo assessments of B. amyloliquefaciens S2.5 on livestock and poultry should be performed to assess its practical application.

Indexed as

AntibioticBacillus amyloliquefaciensGenome-sequencingPotential probioticSpore former

Identifiers

PMID39179321
PMCPMC11338101

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.