Evidence map›Paper›PMID 39178199›Full record

ArticleShock (Augusta, Ga.)2024

POSTINJURY PNEUMONIA INDUCES A UNIQUE BLOOD MICROBIOME SIGNATURE.

Jennifer A Munley, Lauren S Kelly, Gwoncheol Park, Erick E Pons, Kolenkode B Kannan, Letita E Bible, Philip A Efron, Ravinder Nagpal, Alicia M Mohr

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Shock (Augusta, Ga.), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Jennifer A MunleyDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.ORCID 0000-0003-2729-7126
Lauren S KellyDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.
Gwoncheol ParkThe Gut Biome Lab, Department of Health, Nutrition, and Food Sciences, Florida State University College of Education, Health, and Human Sciences, Tallahassee, Florida.
Erick E PonsDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.
Kolenkode B KannanDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.
Letita E BibleDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.
Philip A EfronDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.
Ravinder NagpalThe Gut Biome Lab, Department of Health, Nutrition, and Food Sciences, Florida State University College of Education, Health, and Human Sciences, Tallahassee, Florida.
Alicia M MohrDepartment of Surgery and Sepsis and Critical Illness Research Center, University of Florida College of Medicine, Gainesville, Florida.ORCID 0000-0003-1732-1313

Funding

Molecular Biology in Burns and TraumaT32GM008721 · NIGMS · UNIVERSITY OF FLORIDA · PI Philip A Efron · 1999 to 2026
$5.0M
Chronic Stress and Anemia Recovery following Major TraumaR01GM105893 · NIGMS · UNIVERSITY OF FLORIDA · PI MOHR, ALICIA M · 2013 to 2021
$3.5M
NIGMS NIH HHS R01 GM105893NIGMS NIH HHS T32 GM008721
6 · The paper itself

Abstract

abstractBackground : Previous preclinical studies have demonstrated a pathobiome after traumatic injury; however, the impact of postinjury sepsis on gut epithelial permeability and bacterial translocation remains unknown. We hypothesized that polytrauma with postinjury pneumonia would result in impaired gut permeability leading to specific blood microbiome arrays. Methods : Male and proestrus female Sprague-Dawley rats were subjected to either polytrauma (PT), PT plus 2-hours daily chronic restraint stress (PT/CS), PT with postinjury day 1 inoculation with pseudomonas pneumonia (PT + PNA), PT/CS + PNA, or naive controls. Whole blood microbiome was measured serially using high-throughput 16S rRNA sequencing and QIIME2 bioinformatics analyses. Microbial diversity was assessed using Chao1/Shannon indices and principle coordinate analysis. Intestinal permeability was evaluated by plasma occludin and lipopolysaccharide-binding protein assays. Results : PT/CS + PNA had increased intestinal permeability compared to uninfected counterparts (PT/CS) with significantly elevated occludin ( P < 0.01). Bacteria was not detected in the blood of naïve controls, PT or PT/CS, but was present in both PT + PNA and PT/CS + PNA on days 2 and 7. The PT/CS + PNA blood biome showed dominance of Streptococcus compared to PT + PNA at day 2 ( P < 0.05). Females PT/CS + PNA had a significant abundance of Staphylococcus at day 2 and Streptococcus at day 7 in the blood biome compared to male counterparts ( P < 0.05). Conclusion : Multicompartmental trauma with postinjury pneumonia results in increased intestinal permeability and bacteremia with a unique blood biome, with sexual dimorphisms evident in the blood biome composition. These findings suggest that postinjury sepsis has clinical significance and could influence outcomes after severe trauma and critical illness.

Indexed as

Rats, Sprague-DawleyAnimalsFemaleMaleMicrobiotaMultiple TraumaPneumoniaRatsRNA, Ribosomal, 16SRNA, Ribosomal, 16S

Identifiers

PMID39178199
PMCPMC12231140

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.