Evidence map›Paper›PMID 39174598›Full record

ArticleScientific reports2024

Long-term blood-free rearing of Anopheles mosquitoes with no effect on fitness, Plasmodium infectivity nor microbiota composition.

Joana Marques, Sofia G Seabra, Inês Almeida, Joana Gomes, Ana Catarina Alves, Henrique Silveira

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Joana MarquesGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal. joana.a.marques@gmail.com.
Sofia G SeabraGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal.
Inês AlmeidaGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal.
Joana GomesGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal.
Ana Catarina AlvesGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal.
Henrique SilveiraGlobal Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, Universidade Nova de Lisboa, IHMT-NOVA, Rua da Junqueira 100, 1349-008, Lisboa, Portugal. hsilveira@ihmt.unl.pt.

Funding

by Fundação para a Ciência e Tecnologia Aga-Khan Development Network FCT-AGAKHAN/541725581/2019Fundação para a Ciência e a Tecnologia DOI 10.54499/CEECIND/00450/2017/CP1415/CT0001Fundação para a Ciência e a Tecnologia GHTM-UID/04413/2020Infravec2 731060
6 · The paper itself

Abstract

Mosquito-borne diseases kill millions of people each year. Therefore, many innovative research and population control strategies are being implemented but, most of them require large-scale production of mosquitoes. Mosquito rearing depends on fresh blood from human donors, experimentation animals or slaughterhouses, which constitutes a strong drawback since high blood quantities are needed, raising ethical and financial constraints. To eliminate blood dependency and the use of experimentation animals, we previously developed BLOODless, a patented diet that represents an important advance towards sustainable mosquito breeding in captivity. BLOODless diet was used to maintain a colony of Anopheles stephensi for 40 generations. Bloodmeal appetite, fitness, Plasmodium berghei infectivity, whole genome sequencing and microbiota were evaluated over time. Here we show that BLOODless can be implemented in Anopheles insectaries since it allows long-term rearing of mosquitoes in captivity, without a detectable effect on their fitness, infectivity, nor on their midgut and salivary microbiota composition.

Indexed as

AnophelesMicrobiotaPlasmodium bergheiAnimalsBloodFemaleHumansMalariaMiceMosquito VectorsAnopheles rearingArtificial dietBLOODlessLong-term blood-free rearing

Identifiers

PMID39174598
PMCPMC11341565

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.