Evidence map›Paper›PMID 39172098›Full record

ArticleAging2024

Notch1 signaling pathway promotes growth and metastasis of gastric cancer via modulating CDH5.

Lingshan Zhou, Yuan Yang, Yuwei Ye, Qian Qiao, Yingying Mi, Hongfang Liu, Ya Zheng, Yuping Wang, Min Liu, Yongning Zhou

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In one paragraph

Article in Aging, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Lingshan ZhouDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Yuan YangDepartment of Gastroenterology, The First Affiliated Hospital, Hengyang Medical School, University of South China, Hengyang 421001, Hunan, China.
Yuwei YeDepartment of Gastroenterology Ward 2, Shanxi Provincial People’s Hospital, Xian 710000, China.
Qian QiaoDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Yingying MiDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Hongfang LiuThe First Clinical Medical College, Lanzhou University, Lanzhou 730000, China.
Ya ZhengDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Yuping WangDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Min LiuDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Yongning ZhouDepartment of Gastroenterology, The First Hospital of Lanzhou University, Lanzhou 730000, China.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

objectiveTo explore the underlying molecular mechanism of Notch1/cadherin 5 (CDH5) pathway in modulating in cell malignant behaviors of gastric cancer (GC).

methodsWe performed bioinformatic analyses to screen the potential target genes of Notch1 from cadherins in GC. Western blot and RT-PCR were conducted to detect CDH5 expression in GC tissues and cells. We utilized chromatin immunoprecipitation (CHIP) assays to assess the interaction of Notch1 with CDH5 gene. The effects of Notch1/CDH5 axis on the proliferation, invasion, migration and vasculogenic mimicry in GC cells were evaluated by EdU, wound healing, transwell, and tubule formation assays.

resultsSignificantly increased CDH5 expression was found in GC tissues compared with paracancerous tissues and associated to clinical stage and poor overall survival (OS) in patients with GC. Notch1 positively regulate the expression of CDH5 in GC cells. CHIP assays validated that CDH5 was a direct target of Notch1. In addition, Notch1 upregulation enhanced the proliferation, migration, invasion and vasculogenic mimicry capacity of GC cells, which could be attenuated by CDH5 silencing.

conclusionsThese results indicated Notch1 upregulation enhanced GC malignant behaviors by triggering CDH5, suggesting that targeting Notch1/CDH5 axis could be a potential therapeutic strategy for GC progression.

Indexed as

Antigens, CDCadherinsCell MovementCell ProliferationGene Expression Regulation, NeoplasticReceptor, Notch1Signal TransductionStomach NeoplasmsCadherin 5Cell Line, TumorFemaleHumansMaleMiddle AgedNeoplasm InvasivenessNeoplasm MetastasisAntigens, CDCadherin 5CadherinsNOTCH1 protein, humanReceptor, Notch1CDH5gastric cancerinvasionmigrationNotch1

Identifiers

PMID39172098
PMCPMC11386911

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.