Evidence map›Paper›PMID 39171991›Full record

ArticleThe Laryngoscope2025

Multiple DNA Viruses and HPV Integration in Inverted Papilloma and Associated Sinonasal Carcinoma.

Maria K Jauhiainen, Lari Pyöriä, Sanna Viitasalo, Ushanandini Mohanraj, Leena-Maija Aaltonen, Maria Söderlund-Venermo, Jaana Hagström, Antti A Mäkitie, Maria F Perdomo, Saku T Sinkkonen

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In one paragraph

Article in The Laryngoscope, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. DNA Virus Detection in Olfactory Neuroblastomas Using Targeted Enrichment NGS.Neuropathology : official journal of the Japanese Society of Neuropathology · 2026
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Maria K JauhiainenDepartment of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.ORCID 0000-0003-2310-039X
Lari PyöriäDepartment of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Sanna ViitasaloDepartment of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.ORCID 0000-0001-5460-5265
Ushanandini MohanrajDepartment of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.ORCID 0000-0002-5336-0603
Leena-Maija AaltonenDepartment of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
Maria Söderlund-VenermoDepartment of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Jaana HagströmDepartment of Pathology, University Hospital of Helsinki, Helsinki, Finland.
Antti A MäkitieDepartment of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.
Maria F PerdomoDepartment of Virology, University of Helsinki and Helsinki University Hospital, Helsinki, Finland.
Saku T SinkkonenDepartment of Otorhinolaryngology - Head and Neck Surgery, Helsinki University Hospital and University of Helsinki, Helsinki, Finland.

Funding

Finska LäkaresällskapetHelsingin ja Uudenmaan Sairaanhoitopiiri TYH2024327Life and Health Medical Support AssociationSigrid Juséliuksen SäätiöSuomen Lääketieteen SäätiöUniversity of Helsinki Doctoral Programme
6 · The paper itself

Abstract

objectivesSinonasal inverted papilloma (IP) has a locally destructive growth pattern, can relapse, and can undergo malignant transformation (IP-associated sinonasal squamous cell carcinoma (IP-SNSCC)). Human papillomaviruses (HPV)-6 and -16 are frequently detected in IPs. To clarify the possible roles of other DNA viruses in IPs, we explored viruses not studied in this context before. With the setting of pre- and post-malignant transformation samples, we investigated HPV genomes in depth to assess the integration of HPV into the human genome and the presence of minor intratypic variants. MATERIALS AND

methodsWe analyzed 35 IP samples representing 28 individuals, of which six had IP-SNSCC. For virus screening, we applied qPCR to detect 16 different DNA viruses in three virus families, comprising herpesviruses, parvoviruses, and polyomaviruses. In addition, targeted next generation sequencing (NGS) was used for detailed HPV analysis.

resultsWe detected herpes-, parvo-, and polyomaviruses in 13/28 (46%) patients, with codetections of multiple viruses in six (21%) patients. NGS revealed HPV16 DNA in 2/6 IP-SNSCC and in their respective earlier benign IP samples, as well as in a plasma sample from one of these patients. HPV6 was detected in two IP samples without subsequent malignant transformation. We identified sequence reads containing junctions of HPV6 and HPV16 and host genome suggestive of viral integration. HPV6 and HPV16 minor intratypic variants were present across pre- and post-malignant transformation, with mostly nonsynonymous mutations.

conclusionsMultiple DNA viruses were present in IPs. HPV16 was detected only in IP-SNSCCs or in tumors that later underwent malignant transformation. LEVEL OF EVIDENCE: 3 Laryngoscope, 135:677-686, 2025.

Indexed as

Carcinoma, Squamous CellDNA VirusesPapilloma, InvertedPapillomavirus InfectionsParanasal Sinus NeoplasmsVirus IntegrationAdultAgedAged, 80 and overDNA, ViralFemaleHumansMaleMiddle AgedPapillomaviridaeDNA, Viralcarcinomaherpesvirusinverted papillomapapillomavirusparvovirussquamous cell of head and neck

Identifiers

PMID39171991
PMCPMC11725690

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.