Evidence map›Paper›PMID 39171380›Full record

ArticleDevelopment (Cambridge, England)2024

VitelloTag: a tool for high-throughput cargo delivery into oocytes.

D Nathaniel Clarke, Akshay Kane, Margherita Perillo, Christopher J Lowe, S Zachary Swartz

Abstract read
In one paragraph

Article in Development (Cambridge, England), 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Signal control during tissue regeneration in adult animals.Nature reviews. Molecular cell biology · 2026
    Review
  3. Evidence for vitellogenin DNA-binding in honey bees.Protein science : a publication of the Protein Society · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

D Nathaniel ClarkeDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142,USA.ORCID 0000-0002-9774-2582
Akshay KaneThe Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.ORCID 0000-0002-6891-5253
Margherita PerilloThe Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.ORCID 0000-0003-0845-507X
Christopher J LoweHopkins Marine Station, Stanford University, Pacific Grove, CA 93950,USA.ORCID 0000-0002-7789-8643
S Zachary SwartzThe Eugene Bell Center for Regenerative Biology and Tissue Engineering, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543-1015, USA.ORCID 0000-0002-3264-6880

Funding

Molecular control of oocyte arrest, meiosis, and the transition to developmentR00HD099315 · NICHD · MARINE BIOLOGICAL LABORATORY · PI SWARTZ, STEVEN ZACHARY · 2022 to 2024
$724k
Investigating mechanisms regulating cell adhesion during tissue remodelingF32GM134577 · NIGMS · MASSACHUSETTS INSTITUTE OF TECHNOLOGY · PI CLARKE, DONALD NATHANIEL · 2019 to 2021
$195k
Chan Zuckerberg BioHubHibbitt FoundationMarine Biological Laboratory||University of ChicagoNICHD NIH HHS R00 HD099315NIGMS NIH HHS F32 GM134577NIH HHSNIH HHS R00HD099315The Eugene Bell Center Endowment Fund
6 · The paper itself

Abstract

Delivering molecular tools into oocytes is essential for developmental and reproductive biology. Microinjection, the conventional method, is equipment intensive, often technically challenging and has a low yield, and is impractical in species with delicate oocytes or restricted spawning seasons. To overcome these limitations, we developed VitelloTag, a cost-effective, high-throughput system using vitellogenin-derived fusion proteins to enable efficient cargo delivery via receptor-mediated endocytosis. We demonstrate its utility by delivering Cas9/sgRNA complexes in two distantly related species for gene knockout.

Indexed as

OocytesVitellogeninsAnimalsCRISPR-Cas SystemsEndocytosisFemaleGene Knockout TechniquesMicroinjectionsZebrafishVitellogeninsCRISPR/Cas9Gene editingOocyte deliveryTransgenesisVitellogenesis

Identifiers

PMID39171380
PMCPMC11423919

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.