Evidence map›Paper›PMID 39169954›Full record

ArticleF1000Research2024

A guide to selecting high-performing antibodies for Synaptotagmin-1 (Uniprot ID P21579) for use in western blot, immunoprecipitation, immunofluorescence and flow cytometry.

Michael S Biddle, Charles Alende, Maryam Fotouhi, Carolyn Jones, Riham Ayoubi, Kathleen Southern, Carl Laflamme, Harvinder Virk, NeuroSGC/YCharOS/EDDU collaborative group, ABIF consortium

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in F1000Research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Michael S BiddleNIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.ORCID https://orcid.org/0000-0002-9853-8815
Charles AlendeDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.ORCID https://orcid.org/0009-0005-4611-6134
Maryam FotouhiDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
Carolyn JonesNIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.
Riham AyoubiDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.
Kathleen SouthernDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.ORCID https://orcid.org/0000-0002-4125-3608
Carl LaflammeDepartment of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Québec, H3A 2B4, Canada.ORCID https://orcid.org/0000-0001-5906-025X
Harvinder VirkNIHR BRC-Respiratory, University of Leicester, Leicester, England, UK.ORCID https://orcid.org/0000-0002-9739-9593
NeuroSGC/YCharOS/EDDU collaborative group
ABIF consortium

Funding

TREAT AD Structural Biology CoreU54AG065187 · NIA · EMORY UNIVERSITY · PI Gregory W Carter, ALLAN I LEVEY · 2019 to 2026
$69.7M
NIA NIH HHS U54 AG065187
6 · The paper itself

Abstract

Synaptotagmin-1 is a synaptic vesicle transmembrane protein that senses calcium influx via its tandem C2-domains, triggering synchronous neurotransmitter release. Disruption to

Indexed as

AntibodiesBlotting, WesternFlow CytometryFluorescent Antibody TechniqueImmunoprecipitationSynaptotagmin IHumansAntibodiesSynaptotagmin Iantibody characterizationantibody validationimmunofluorescenceimmunoprecipitationSynaptotagmin-1SYT1Uniprot ID P21579western blot

Identifiers

PMID39169954
PMCPMC11336552

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.