Evidence map›Paper›PMID 39169077›Full record

ArticleScientific reports2024

Rapid function analysis of OsiWAK1 using a Dual-Luciferase assay in rice.

Wuzhong Yin, Hongxia Yang, Ping Feng, Pan Qi, Biluo Li, Yuanyuan Li, Qingxiong Huang, Youlin Peng, Nan Wang, Yungao Hu

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In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Wuzhong Yin *College of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Hongxia Yang *Key Laboratory of Application and Safety Control of Genetically Modified Crops, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
Ping FengKey Laboratory of Application and Safety Control of Genetically Modified Crops, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China.
Pan QiCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Biluo LiCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Yuanyuan LiCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Qingxiong HuangCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Youlin PengCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China.
Nan WangKey Laboratory of Application and Safety Control of Genetically Modified Crops, College of Agronomy and Biotechnology, Southwest University, Chongqing, 400715, China. wangnan_xndx@126.com.
Yungao HuCollege of Life Science and Engineering, Southwest University of Science and Technology, Mianyang, 621010, China. huyungao@swust.edu.cn.

Funding

Fund Projects for Local Science and Technology Development Guided by China Government 2022ZYDF078Natural Science Foundation of Southwest University of Science and Technology 22zx7144Sichuan Province Science and Technology Support Program 2023NSFSC1170the Key Research and Development Program of Sichuan 2021YFYZ0016the Major Science and Technology Projects in Sichuan Province 2022ZDZX0012
6 · The paper itself

Abstract

In the past decade, the exploration of genetic resources in rice has significantly enhanced the efficacy of rice breeding. However, the exploration of genetic resources is hindered by the identification of candidate genes. To expedite the identification of candidate genes, this study examined tapetum programmed cell death-related genes OsiWAK1, OsPDT1, EAT1, TDR, and TIP2 to assess the efficacy of the Dual-Luciferase (Dual-LUC) assay in rapidly determining gene relationships. The study found that, in the Dual-LUC assay, OsiWAK1 and its various recombinant proteins exhibit comparable activation abilities on the EAT1 promoter, potentially indicating a false positive. However, the Dual-LUC assay can reveal that OsiWAK1 impacts both the function of its upstream regulatory factor OsPDT1 and the TDR/TIP2 transcription complex. By rapidly studying the relationship between diverse candidate genes and regulatory genes in a well-known trait via the Dual-LUC assay, this study provides a novel approach to expedite the determination of candidate genes such as genome-wide association study.

Indexed as

Gene Expression Regulation, PlantOryzaPlant ProteinsLuciferasesPromoter Regions, GeneticLuciferasesPlant ProteinsCandidate genes researchDual-Luciferase assayGene function analysisRiceWall associated kinase

Identifiers

PMID39169077
PMCPMC11339413

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.