Article in Nature communications, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
0numbers the graph read from it
0cells of the map it votes in
7citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registry
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Sarah R HengelUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA. sarah.hengel@tufts.edu.ORCID 0000-0002-0408-8623
Katherine G OppenheimerUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.ORCID 0000-0002-3809-4044
Chelsea M SmithUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.ORCID 0000-0001-8263-9817
Matthew A SchaichUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.
Hayley L ReinUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.
Julieta MartinoUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.
Kristie E DarrahUniversity of Pennsylvania School of Medicine, Penn Center for Genome Integrity, Department of Biochemistry and Biophysics, 421 Curie Boulevard, Philadelphia, PA, USA.
Oluchi C EzekwennaTufts University, Department of Biology, Medford, MA, USA.ORCID 0009-0003-1428-1544
Kyle R BurtonTufts University, Department of Biology, Medford, MA, USA.
Bennett Van HoutenUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA.ORCID 0000-0002-4009-2478
Maria SpiesUniversity of Iowa, Department of Biochemistry and Molecular Biology, Iowa City, IA, USA.ORCID 0000-0002-7375-8037
Kara A BernsteinUniversity of Pittsburgh, School of Medicine, Department of Pharmacology and Chemical Biology, UPMC-Hillman Cancer Center, Pittsburgh, PA, USA. kara.bernstein@pennmedicine.upenn.edu.ORCID 0000-0003-2247-6459
Funding
VECTOR CORE FACILITYP30CA047904 · NCI · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI CHRISTOPHER J. BAKKENIST · 1988 to 2026
$158.0M
Viral VectorP30CA086862 · NCI · UNIVERSITY OF IOWA · PI Jon C.D. Houtman · 2000 to 2026
$70.0M
Translational Research Support CoreP30ES013508 · NIEHS · UNIVERSITY OF PENNSYLVANIA · PI A. Clementina Mesaros · 2006 to 2026
$35.3M
Watching cooperative interactions between base and nucleotide excision repair proteinsR35ES031638 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Bennett Van Houten · 2020 to 2026
$6.2M
Assembly and Dynamics of Molecular Machines in Genome MaintenanceR35GM131704 · NIGMS · UNIVERSITY OF IOWA · PI Maria Spies · 2019 to 2026
$3.9M
Replication fork dynamics and repair by Rad51 paralogues after DNA alkylationR01ES030335 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI Kara A Bernstein · 2019 to 2026
$2.9M
RAD51 paralog function in cancer predisposition and genome integrity R01ES031796 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI BERNSTEIN, KARA A · 2021 to 2025
$2.2M
Shu complex and RAD52 function in DNA damage recognition and subsequent repairR00ES033738 · NIEHS · TUFTS UNIVERSITY MEDFORD · PI HENGEL, SARAH R · 2023 to 2025
$747k
Lumicks C-Trap Optical Tweezers with Confocal Fluorescence MicroscopeS10OD032158 · OD · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI VAN HOUTEN, BENNETT · 2023 to 2023
$552k
Shu complex and RAD52 function in DNA damage recognition and subsequent repairK99ES033738 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI HENGEL, SARAH R · 2022 to 2023
$216k
DNA repair pathway coordination during damage processingF32ES034982 · NIEHS · UNIVERSITY OF PITTSBURGH AT PITTSBURGH · PI SCHAICH, MATTHEW ALLEN · 2023 to 2025
$131k
NCI NIH HHS P30 CA047904NCI NIH HHS P30 CA086862NIEHS NIH HHS F32 ES034982NIEHS NIH HHS K99 ES033738NIEHS NIH HHS P30 ES013508NIEHS NIH HHS R00 ES033738NIEHS NIH HHS R01 ES030335NIEHS NIH HHS R01 ES031796NIEHS NIH HHS R35 ES031638NIGMS NIH HHS R35 GM131704NIH HHS S10 OD032158U.S. Department of Health & Human Services | National Institutes of Health (NIH) ES030335U.S. Department of Health & Human Services | National Institutes of Health (NIH) ES031796U.S. Department of Health & Human Services | National Institutes of Health (NIH) ES033738U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) 1S10OD032158-01A1U.S. Department of Health & Human Services | NIH | National Institute of Environmental Health Sciences (NIEHS) R35ES031638U.S. Department of Health & Human Services | NIH | National Institute of General Medical Sciences (NIGMS) R35GM131704
6 · The paper itself
Abstract
Templated DNA repair that occurs during homologous recombination and replication stress relies on RAD51. RAD51 activity is positively regulated by BRCA2 and the RAD51 paralogs. The Shu complex is a RAD51 paralog-containing complex consisting of SWSAP1, SWS1, and SPIDR. We demonstrate that SWSAP1-SWS1 binds RAD51, maintains RAD51 filament stability, and enables strand exchange. Using single-molecule confocal fluorescence microscopy combined with optical tweezers, we show that SWSAP1-SWS1 decorates RAD51 filaments proficient for homologous recombination. We also find SWSAP1-SWS1 enhances RPA diffusion on ssDNA. Importantly, we show human sgSWSAP1 and sgSWS1 knockout cells are sensitive to pharmacological inhibition of PARP and APE1. Lastly, we identify cancer variants in SWSAP1 that alter Shu complex formation. Together, we show that SWSAP1-SWS1 stimulates RAD51-dependent high-fidelity repair and may be an important new cancer therapeutic target.
Indexed as
DNA, Single-StrandedRad51 RecombinaseReplication Protein ADNA-(Apurinic or Apyrimidinic Site) LyaseDNA-Binding ProteinsDNA RepairHomologous RecombinationHumansPoly(ADP-ribose) Polymerase InhibitorsProtein BindingSingle Molecule ImagingDNA-(Apurinic or Apyrimidinic Site) LyaseDNA-Binding ProteinsDNA, Single-StrandedPoly(ADP-ribose) Polymerase InhibitorsRAD51 protein, humanRad51 RecombinaseReplication Protein ASWSAP1 protein, humanZSWIM7 protein, human
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.
The human Shu complex promotes RAD51 activity by modulating RPA dynamics on ssDNA. · full record | OpenQuestion