ArticleScience advances2024
Click-free imaging of carbohydrate trafficking in live cells using an azido photothermal probe.
Article in Science advances, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
9 citing papers in PubMed.
- FILM: mapping organellar metabolism by mid-infrared photothermal-modulated fluorescence.Nature methods · 2026Article
- FILM: Mapping organellar metabolism by mid-infrared photothermal modulated fluorescence.ArXiv · 2026Article
- Feeling the vibes: Vibrational spectroscopic imaging of biomolecular assemblies in their natural environment.Applied physics reviews · 2025Article
- Ultrasensitive in vivo infrared spectroscopic imaging via oblique photothermal microscopy.Nature communications · 2025Article
- Advanced vibrational microscopes for life science.Nature methods · 2025Review
- A 20-year journey on the invention of vibrational photothermal microscopy.Nature methods · 2025Article
- Optical Photothermal Infrared Imaging Using Metabolic Probes in Biological Systems.Analytical chemistry · 2025Article
- Background-Free Mid-Infrared Photothermal Microscopy via Single-Shot Measurement of Thermal Decay.Analytical chemistry · 2025Article
- Mid-infrared Photothermal Imaging: Instrument and Life Science Applications.Analytical chemistry · 2024Review
Corrections and comments
- Update of
Authors and funding
13 authors.
Funding
Abstract
Real-time tracking of intracellular carbohydrates remains challenging. While click chemistry allows bio-orthogonal tagging with fluorescent probes, the reaction permanently alters the target molecule and only allows a single snapshot. Here, we demonstrate click-free mid-infrared photothermal (MIP) imaging of azide-tagged carbohydrates in live cells. Leveraging the micromolar detection sensitivity for 6-azido-trehalose (TreAz) and the 300-nm spatial resolution of MIP imaging, the trehalose recycling pathway in single mycobacteria, from cytoplasmic uptake to membrane localization, is directly visualized. A peak shift of azide in MIP spectrum further uncovers interactions between TreAz and intracellular protein. MIP mapping of unreacted azide after click reaction reveals click chemistry heterogeneity within a bacterium. Broader applications of azido photothermal probes to visualize the initial steps of the Leloir pathway in yeasts and the newly synthesized glycans in mammalian cells are demonstrated.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.