Evidence map›Paper›PMID 39167044›Full record

ArticleACS sensors2024

Development of a Dual Reporter System to Simultaneously Visualize Ca

Yusuf C Erdoğan, Johannes Pilic, Benjamin Gottschalk, Esra N Yiğit, Asal G Zaki, Gürkan Öztürk, Emrah Eroğlu, Begüm Okutan, Nicole G Sommer, Annelie M Weinberg and 3 more

Abstract read
In one paragraph

Article in ACS sensors, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Review
  2. Article
  3. Review
  4. Chemical reviews · 2026
    Review
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yusuf C ErdoğanGottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Neue Stiftingtalstraße 6, Graz 8010, Austria.
Johannes PilicGottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Neue Stiftingtalstraße 6, Graz 8010, Austria.
Benjamin GottschalkGottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Neue Stiftingtalstraße 6, Graz 8010, Austria.
Esra N YiğitRegenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul 34810, Turkey.
Asal G ZakiRegenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul 34810, Turkey.
Gürkan ÖztürkRegenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul 34810, Turkey.ORCID 0000-0003-0352-1947
Emrah EroğluRegenerative and Restorative Medicine Research Center (REMER), Research Institute for Health Sciences and Technologies (SABITA), Istanbul Medipol University, Istanbul 34810, Turkey.
Begüm OkutanDepartment of Orthopedics and Traumatology, Medical University of Graz, Auenbruggerplatz 5, Graz 8036, Austria.ORCID 0000-0002-4951-5940
Nicole G SommerDepartment of Orthopedics and Traumatology, Medical University of Graz, Auenbruggerplatz 5, Graz 8036, Austria.
Annelie M WeinbergDepartment of Orthopedics and Traumatology, Medical University of Graz, Auenbruggerplatz 5, Graz 8036, Austria.
Rainer SchindlGottfried Schatz Research Center, Biophysics, Medical University of Graz, Neue Stiftingtalstrasse 6, Graz 8010, Austria.
Wolfgang F GraierGottfried Schatz Research Center, Molecular Biology and Biochemistry, Medical University of Graz, Neue Stiftingtalstraße 6, Graz 8010, Austria.ORCID 0000-0003-1871-3298
Roland MalliBioTechMed Graz, Mozartgasse 12/2, Graz 8010, Austria.ORCID 0000-0001-6327-8729

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In this study, we introduce a new separation of phases-based activity reporter of kinase (SPARK) for AMP-activated kinase (AMPK), named AMPK-SPARK, which reports the AMPK activation by forming bright fluorescent clusters. Furthermore, we introduce a dual reporter system, named GCaMP-AMPK-SPARK, by incorporating a single-fluorescent protein (FP)-based Ca

Indexed as

AMP-Activated Protein KinasesBiosensing TechniquesCalciumCalcium SignalingAnimalsEnzyme ActivationHEK293 CellsHumansMicroscopy, FluorescenceAMP-Activated Protein KinasesCalciumAMPK reporterCa2+ biosensorfluorescence microscopyGCaMPmultiplexingphase separation-based activity reporter of kinaseSPARK

Identifiers

PMID39167044
PMCPMC11443530

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.