ArticlePlant biotechnology journal2024
DEAD-box RNA helicase RH20 positively regulates RNAi-based antiviral immunity in plants by associating with SGS3/RDR6 bodies.
Article in Plant biotechnology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- The dehydrin protein COR15 enhances antiviral RNA silencing by preventing viral protein-mediated SGS3 degradation and promoting SGS3 condensate formation.Plant communications · 2026Article
- Silencing Suppressor Protein p26 of Areca Palm Velarivirus 1 (APV1) Interacts With SGS3 and Promotes Its Degradation Via the Ubiquitination Pathway.Molecular plant pathology · 2026Article
- Methionine Synthase Positively Regulates Plant Defence to Both RNA and DNA Viruses and Is Useful for Developing Broad-Spectrum Antiviral Resistance in Crops.Plant biotechnology journal · 2026Article
- Liquid-liquid phase separation at the frontline of plant-pathogen battles.Plant communications · 2026Review
- First genomic insights into the introgression of almond PPV-Marcus resistance into peach.Frontiers in plant science · 2026Article
- RING-Between-RING-Type E3 Ligase Ariadne-Like Protein 8 Negatively Regulates Plant Virus Infection by Targeting a Viral Movement Protein.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Review
- Antiviral RNA interference in plants: Increasing complexity and integration with other biological processes.Plant communications · 2025Review
- The MC4-La1 cleavage module restricts plant virus infection by integrating R-motif-mediated defense mRNA translation.Science advances · 2025Article
- Advances in understanding multifunctionality of Barley stripe mosaic virus γb protein.PLoS pathogens · 2025Review
- A phosphorylation-driven ubiquitination switch fine-tunes Alfin-like 7-induced ROS signaling in plant immunity.Science advances · 2025Article
- A cell wall-associated kinase phosphorylates NLR immune receptor to negatively regulate resistosome formation.Nature plants · 2025Article
- Small RNA, big defence: Early epigenetic responses to genetic invasion.Quantitative plant biology · 2025Review
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Authors and funding
14 authors.
Funding
Abstract
RNA silencing plays a crucial role in defending against viral infections in diverse eukaryotic hosts. Despite extensive studies on core components of the antiviral RNAi pathway such as DCLs, AGOs and RDRs proteins, host factors involved in antiviral RNAi remain incompletely understood. In this study, we employed the proximity labelling approach to identify the host factors required for antiviral RNAi in Nicotiana benthamiana. Using the barley stripe mosaic virus (BSMV)-encoded γb, a viral suppressor of RNA silencing (VSR), as the bait protein, we identified the DEAD-box RNA helicase RH20, a broadly conserved protein in plants and animals with a homologous human protein known as DDX5. We demonstrated the interaction between RH20 and BSMV γb. Knockdown or knockout of RH20 attenuates the accumulation of viral small interfering RNAs, leading to increased susceptibility to BSMV, while overexpression of RH20 enhances resistance to BSMV, a process requiring the cytoplasmic localization and RNA-binding activity of RH20. In addition to BSMV, RH20 also negatively regulates the infection of several other positive-sense RNA viruses, suggesting the broad-spectrum antiviral activity of RH20. Mechanistic analysis revealed the colocalization and interaction of RH20 with SGS3/RDR6, and disruption of either SGS3 or RDR6 undermines the antiviral function of RH20, suggesting RH20 as a new component of the SGS3/RDR6 bodies. As a counter-defence, BSMV γb VSR subverts the RH20-mediated antiviral defence by interfering with the RH20-SGS3 interaction. Our results uncover RH20 as a new positive regulator of antiviral RNAi and provide new potential targets for controlling plant viral diseases.
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