Evidence map›Paper›PMID 39164737›Full record

ArticleCell communication and signaling : CCS2024

ZMAT2 condensates regulate the alternative splicing of TRIM28 to reduce cellular ROS accumulation, thereby promoting the proliferation of HCC cells.

Yaning Zhu, Jiong Li, Sang Li, Zhe Yang, Zhengkang Qiao, Xingshi Gu, Zhenhu He, Di Wu, Xiaoqian Ma, Shanhu Yao and 6 more

Abstract read
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In one paragraph

Article in Cell communication and signaling : CCS, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

16 authors.

Yaning Zhu *Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Jiong Li *Department of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Sang LiEngineering and Technology Research Center for Xenotransplantation of Human Province, Changsha, China.
Zhe YangCollege of Life Science, Liaoning University, Shenyang, China.
Zhengkang QiaoCollege of Life Science, Liaoning University, Shenyang, China.
Xingshi GuDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Zhenhu HeDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Di WuDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Xiaoqian MaDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Shanhu YaoDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Cejun YangDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Min YangDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Lu CaoDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Juan ZhangDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Wei WangDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China.
Pengfei RongDepartment of Radiology, The Third Xiangya Hospital, Central South University, Changsha, Hunan, China. rongpengfei66@163.com.

Funding

National Outstanding Youth Science Fund Project of National Natural Science Foundation of China 31800688the Foundation of Liaoning Educational Committee of China JYTMS20230765the Furong Scholars Programme of Hunan Province 2022the Key Research and Development Program of Hunan Province 2022SK2025The National Natural Science Foundation of China 82071986The National Natural Science Foundation of China 82372071the Science and Technology Project of Hunan Province 2021RC4017The Science and Technology Project of Hunan Province 2023JJ40882The Science and Technology Project of Hunan Province 2024JJ6624the Screening of small-molecule tumor suppression compounds project SDUZHHT〔2022〕2448the Shenyang Youth Science and Technology Innovation Project RC220177the Wisdom Accumulation and Talent Cultivation Project of the Third Xiangya Hospital of Central South University 2020
6 · The paper itself

Abstract

Dysregulation of splicing factor expression plays a crucial role in the progression of hepatocellular carcinoma (HCC). Our research found that the expression level of splicing factor ZMAT2 was increased in HCC, promoting the proliferation of HCC cells. RNAseq data indicated that the absence of ZMAT2 induced skipping exon of mRNA, while RIPseq data further revealed the mRNA binding motifs of ZMAT2. A comprehensive analysis of RNAseq and RIPseq data indicateed that ZMAT2 played a crucial role in the maturation process of TRIM28 mRNA. Knocking down of ZMAT2 led to the deletion of 25 bases in exon 11 of TRIM28, ultimately resulting in nonsense-mediated decay (NMD). Our data revealed that ZMAT2 could regulate TRIM28 to reduce the accumulation of ROS in HCC cells, thereby promoting their proliferation. Our research also discovered that ZMAT2 was capable of undergoing phase separation, resulting in the formation of liquid droplet condensates within HCC cells. Additionally, it was found that ZMAT2 was able to form protein-nucleic acid condensates with TRIM28 mRNA. In summary, this study is the first to reveal that ZMAT2 and TRIM28 mRNA form protein-nucleic acid condensates, thereby regulating the splicing of TRIM28 mRNA. The increased expression of ZMAT2 in HCC leads to upregulated TRIM28 expression and reduced ROS accumulation, ultimately accelerating the proliferation of HCC cells.

Indexed as

Alternative SplicingCarcinoma, HepatocellularCell ProliferationLiver NeoplasmsReactive Oxygen SpeciesTripartite Motif-Containing Protein 28Cell Line, TumorGene Expression Regulation, NeoplasticHumansRNA-Binding ProteinsRNA, MessengerReactive Oxygen SpeciesRNA-Binding ProteinsRNA, MessengerTRIM28 protein, humanTripartite Motif-Containing Protein 28HCCPhase separationProliferationROSTRIM28ZMAT2

Identifiers

PMID39164737
PMCPMC11337747

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.