Evidence map›Paper›PMID 39164317›Full record

ArticleScientific reports2024

Development and validation of sex-linked molecular markers for rapid and accurate identification of male and female Hippophae tibetana plants.

Zhefei Zeng, Ruoqiu Wang, Junwei Wang, Yonghao Chen, Yuguo Wang, Zhiping Song, Wenju Zhang, La Qiong

Abstract read
In one paragraph

Article in Scientific reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

Who cites it

6 citing papers in PubMed.

  1. Nanopore Data-Driven Near-T2T Genome Assembly ofPlants (Basel, Switzerland) · 2026
    Article
  2. Article
  3. Article
  4. Article
  5. Genetic diversity ofFrontiers in plant science · 2025
    Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Zhefei ZengKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, School of Ecology and Environment, Tibet University, Lhasa, 850000, China.
Ruoqiu WangTech X Academy, Shenzhen Polytechnic University, Shenzhen, 518055, China.
Junwei WangKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, School of Ecology and Environment, Tibet University, Lhasa, 850000, China.
Yonghao ChenKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, School of Ecology and Environment, Tibet University, Lhasa, 850000, China.
Yuguo WangTibet University-Fudan University Joint Laboratory for Biodiversity and Global Change, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Zhiping SongTibet University-Fudan University Joint Laboratory for Biodiversity and Global Change, School of Life Sciences, Fudan University, Shanghai, 200438, China.
Wenju ZhangTibet University-Fudan University Joint Laboratory for Biodiversity and Global Change, School of Life Sciences, Fudan University, Shanghai, 200438, China. wjzhang@fudan.edu.cn.
La QiongKey Laboratory of Biodiversity and Environment on the Qinghai-Tibetan Plateau, Ministry of Education, School of Ecology and Environment, Tibet University, Lhasa, 850000, China. lhagchong@163.com.

Funding

First-class Discipline Construction Project of Ecology Zangcaijiaozhi [2023]01Graduate High-level Talent Training Program of Tibet University 2022-GSP-B003Key R&D Project of Science and Technology Program of Tibet Autonomous Region XZ202301ZY0006GNational Natural Science Foundation of China 31760127Tibet Autonomous Region Natural Science Foundation Project ZRKX2024000019
6 · The paper itself

Abstract

Hippophae tibetana, one of the highest-altitude woody plants endemic to the Qinghai-Tibet Plateau, primarily thrives on riverbanks formed by glacial meltwater. As a dioecious species, it demonstrates significant ecological and economic value in extreme alpine environments. However, the lack of sex identification techniques outside of the flowering period severely limits research on sex ratio, differentiation, and breeding. There is an urgent need to develop effective sex-linked molecular markers that are independent of developmental stages, but current research in this area remains limited. This study developed a set of accurate sex-linked molecular markers for the rapid identification of male and female individuals of H. tibetana. Through whole-genome resequencing of 32 sexually differentiated H. tibetana samples, this study offers strong evidence supporting chromosome 2 as the sex chromosome and successfully identified key loci related to sex determination on this chromosome. Utilizing these loci, we, for the first time, developed three reliable pairs of sex-specific molecular markers, which exhibited high accuracy during validation across various geographic populations, offering an effective tool for the sex identification of H. tibetana. Additionally, this study lays the groundwork for further research into the mechanisms of sex determination and the evolution of sex chromosomes in H. tibetana.

Indexed as

Sex ChromosomesChromosomes, PlantGenetic MarkersGenome, PlantSex Determination ProcessesTibetGenetic MarkersHippophae tibetanaMolecular markersSex chromosomesSex determinationWhole-genome resequencing

Identifiers

PMID39164317
PMCPMC11336215

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