ArticleJournal of proteome research2024
An Inflection Point in High-Throughput Proteomics with Orbitrap Astral: Analysis of Biofluids, Cells, and Tissues.
Article in Journal of proteome research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
22 citing papers in PubMed.
- Time to rethink circadian rhythms beyond the transcriptome.Journal of biochemistry · 2026Review
- Immunoglobulin sub-class levels define inter-donor plasma variability: a longitudinal dual-lab study.Molecular systems biology · 2026Article
- Limited Impact of Column Chemistry and Length on Proteome Coverage Under High-Speed DIA.Molecular & cellular proteomics : MCP · 2026Article
- A Parallel Accumulation-Mobility Aligned Fragmentation Strategy Utilizing High-Resolution Ion Mobility for High-Performance Proteomics Analysis.Molecular & cellular proteomics : MCP · 2026Article
- Consensus statement on mass spectrometry-based proteomic analysis of cardiac tissue.Nature cardiovascular research · 2026Article
- What Are the Practical Applications of Single-Cell Proteomics?Proteomes · 2026Article
- Environmental Regulation, Molecular Profiling, and Preliminary Functional Evaluation of Extracellular Vesicles fromFoods (Basel, Switzerland) · 2026Article
- Impact of Variations in Commercial Standard Operating Procedure on Plasma Proteome Recovery.Journal of proteome research · 2026Article
- Harnessing New Tools for Old Challenges: Optimising Neat Plasma Proteomics with Automation and Gas-Phase Fractionation.ACS measurement science au · 2026Article
- Penicillin-Streptomycin Treatment Rewires Core Metabolic and Ribosomal Programs in HepG2 Cells.Journal of proteome research · 2026Article
- Asporin Improves Cardiac Myocyte Response to Ischemia and Reperfusion Stress.bioRxiv : the preprint server for biology · 2026Article
- Performance Characteristics of Zeno Trap Scanning DIA for Sensitive and Quantitative Proteomics at High Throughput.Proteomics · 2026Article
- Comparative evaluation of Olink Explore 3072 and mass spectrometry with peptide fractionation for plasma proteomics.Communications chemistry · 2025Article
- Pre-analytical drivers of bias in bead-enriched plasma proteomics.EMBO molecular medicine · 2025Article
- Current landscape of plasma proteomics from technical innovations to biological insights and biomarker discovery.Communications chemistry · 2025Article
- Thin-diaPASEF: diaPASEF for maximizing proteome coverage in single-shot proteomics.DNA research : an international journal for rapid publication of reports on genes and genomes · 2025Article
- Development of an Adaptive, Economical, and Easy-to-Use SP3-TMT Automated Sample Preparation Workflow for Quantitative Proteomics.Journal of proteome research · 2025Article
- TMT-Based Multiplexed (Chemo)Proteomics on the Orbitrap Astral Mass Spectrometer.Molecular & cellular proteomics : MCP · 2025Article
- Integrating -Omic Technologies across Modality, Space, and Time to Decipher Remodeling in Cardiac Disease.Current cardiology reports · 2025Review
- Recent Advances in Mass Spectrometry-Based Bottom-Up Proteomics.Analytical chemistry · 2025Review
Corrections and comments
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Authors and funding
11 authors.
Funding
Abstract
This Technical Note presents a comprehensive proteomics workflow for the new combination of Orbitrap and Astral mass analyzers across biofluids, cells, and tissues. Central to our workflow is the integration of Adaptive Focused Acoustics (AFA) technology for cells and tissue lysis to ensure robust and reproducible sample preparation in a high-throughput manner. Furthermore, we automated the detergent-compatible single-pot, solid-phase-enhanced sample Preparation (SP3) method for protein digestion. The synergy of these advanced methodologies facilitates a robust and high-throughput approach for cell and tissue analysis, an important consideration in translational research. This work disseminates our platform workflow, analyzes the effectiveness, demonstrates the reproducibility of the results, and highlights the potential of these technologies in biomarker discovery and disease pathology. For cells and tissues (heart, liver, lung, and intestine) proteomics analysis by data-independent acquisition mode, identifications exceeding 10,000 proteins can be achieved with a 24 min active gradient. In 200 ng injections of HeLa digest across multiple gradients, an average of more than 80% of proteins have a CV less than 20%, and a 45 min run covers ∼90% of the expressed proteome. This complete workflow allows for large swaths of the proteome to be identified and is compatible with diverse sample types.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.