Evidence map›Paper›PMID 39163113›Full record

ArticleThe Journal of general virology2024

The effect of pH on the structure of Bluetongue virus VP5.

Hanwen Zhang, Kamel El Omari, Geoff Sutton, David I Stuart

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of general virology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Hanwen ZhangDivision of Structural Biology, University of Oxford, Centre for Human Genetics, Oxford, UK.
Kamel El OmariDivision of Structural Biology, University of Oxford, Centre for Human Genetics, Oxford, UK.
Geoff SuttonDivision of Structural Biology, University of Oxford, Centre for Human Genetics, Oxford, UK.
David I StuartDivision of Structural Biology, University of Oxford, Centre for Human Genetics, Oxford, UK.

Funding

Medical Research CouncilWellcome Trust
6 · The paper itself

Abstract

The unenveloped Bluetongue virus capsid comprises several structural layers, the inner two comprising a core, which assembles before addition of the outer proteins, VP2 and VP5. Two symmetric trimers of VP5 fit like pegs into two distinct pits on the core and undergo pH conformational changes in the context of the virus, associated with cell entry. Here we show that in isolation VP5 alone undergoes essentially the same changes with pH and confirm a helical transition, indicating that VP5 is a motor during cell entry. In the absence of VP5 the two pits on the core differ from each other, presumably due to the asymmetric underlying structure of VP3, the innermost capsid protein. On insertion of VP5 these pits become closely similar and remain similar at low pH whilst VP5 is present. This natural asymmetry presumably destabilises the attachment of VP5, facilitating ejection upon low pH, membrane penetration and cell entry.

Indexed as

Bluetongue virusCapsid ProteinsAnimalsHydrogen-Ion ConcentrationProtein ConformationVirus InternalizationCapsid ProteinsVP5 protein, Bluetongue virusBluetongue viruspH effectvirus capsid structurevirus entryVP5X-ray crystallography

Identifiers

PMID39163113
PMCPMC11335306

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.