Evidence map›Paper›PMID 39162967›Full record

ArticleProbiotics and antimicrobial proteins2025

Physiological and Technological Properties of Probiotic Lacticaseibacillus rhamnosus GG Encapsulated with Alginate-Chitosan Mixture and Its Incorporation in Whole Milk.

Ana Júlia Alves Aguiar Athayde, Lúcia Raquel Ramos Berger, Thatyane Mariano Rodrigues de Albuquerque, Karoliny Brito Sampaio, Karina Félix Dias Fernandes, Heloisa Maria Almeida do Nascimento, Sônia Paula Alexandrino de Oliveira, Laênia Angélica Andrade Lopes, Carlos Eduardo Vasconcelos de Oliveira, Maria Lúcia da Conceição and 2 more

Abstract read
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In one paragraph

Article in Probiotics and antimicrobial proteins, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Whole genome sequencing analysis and functional characterization of Lacticaseibacillus rhamnosus HP-B1083.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2026
    Article
  2. Review
  3. Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors.

Ana Júlia Alves Aguiar AthaydePost-Graduate Program in Nutrition, Health Sciences Center, Federal University of Pernambuco, Recife, PE, Brazil.
Lúcia Raquel Ramos BergerLaboratory of Agricultural and Environmental Microbiology, Department of Biology, Federal Rural University of Pernambuco, Recife, PE, Brazil.
Thatyane Mariano Rodrigues de AlbuquerqueLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil. thatyane.albuquerque3@academico.ufpb.br.
Karoliny Brito SampaioLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Karina Félix Dias FernandesLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Heloisa Maria Almeida do NascimentoLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Sônia Paula Alexandrino de OliveiraLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Laênia Angélica Andrade LopesDepartment of Nutrition, College of São Francisco of Paraíba, Campina Grande, PB, Brazil.
Carlos Eduardo Vasconcelos de OliveiraLaboratory of Bromatology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Maria Lúcia da ConceiçãoLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Evandro Leite de SouzaLaboratory of Food Microbiology, Department of Nutrition, Federal University of Paraíba, João Pessoa, PB, Brazil.
Thayza Christina Montenegro StamfordPost-Graduate Program in Nutrition, Health Sciences Center, Federal University of Pernambuco, Recife, PE, Brazil.

Funding

Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq, Brazil Process n° 300885/2021-0Fundação de Amparo a Ciência e Tecnologia do Estado de Pernambuco - FACEPE APQ-0263-2.12/19
6 · The paper itself

Abstract

This study developed and evaluated chitosan-sodium alginate capsules containing the probiotic Lacticaseibacillus rhamnosus GG using extrusion and emulsification techniques. The encapsulated L. rhamnosus GG cells were also evaluated for technological and probiotic-related physiological functionalities, as well as when incorporated in UHT and powdered milk. Extrusion (86.01 ± 1.26%) and emulsification (74.43 ± 1.41%) encapsulation techniques showed high encapsulation efficiency and high survival rates of L. rhamnosus GG during 28 days of refrigeration and room temperature storage, especially emulsification capsules (> 81%). The encapsulated L. rhamnosus GG cells showed high survival rates during exposure to simulated gastrointestinal conditions (72.65 ± 1.09-114.15 ± 0.44%). L. rhamnosus GG encapsulated by extrusion and emulsification performed satisfactorily in probiotic-related physiological (pH and bile salts tolerance) and technological properties (positive proteolytic activity, diacetyl and exopolysaccharides production, high NaCl tolerance (> 91%), besides having high heat tolerance (> 76%)). L. rhamnosus GG in extrusion and emulsification capsules had high survival rates (> 89%) and did not significantly affect physicochemical parameters in Ultra-High Temperature (UHT) and powdered milk during storage. The results demonstrate that L. rhamnosus GG can be successfully encapsulated with alginate-chitosan as a protective material through extrusion and emulsification techniques. UHT and powdered milk could serve as appropriate delivery systems to increase the intake of this encapsulated probiotic by consumers.

Indexed as

AlginatesChitosanLacticaseibacillus rhamnosusMilkProbioticsAnimalsCapsulesDrug CompoundingAlginatesCapsulesChitosanDeliveryDigestionEncapsulationPowdered milkStorageSurvivalUHT milk

Identifiers

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.