Evidence map›Paper›PMID 39162514›Full record

ArticleMicrobiology spectrum2024

Development of an inducer-free, virulence gene promoter-controlled, and fluorescent reporter-labeled CRISPR interference system in

Roni Miah, Mona Johannessen, Morten Kjos, Christian S Lentz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Roni MiahDepartment of Medical Biology and Center for New Antibacterial Strategies (CANS), UT- The Arctic University of Norway, Tromsø, Norway.ORCID 0009-0002-1044-9269
Mona JohannessenDepartment of Medical Biology and Center for New Antibacterial Strategies (CANS), UT- The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0003-4604-8600
Morten KjosFaculty of Chemistry, Biotechnology and Food Science, Norwegian University of Life Sciences, Ås, Norway.ORCID 0000-0003-4448-9082
Christian S LentzDepartment of Medical Biology and Center for New Antibacterial Strategies (CANS), UT- The Arctic University of Norway, Tromsø, Norway.ORCID 0000-0001-7284-2264

Funding

Centre for New Antibacterial Strategies (CANS) Start-up grantJoint Programming Initiative on Antimicrobial Resistance (JPIAMR) 296906
6 · The paper itself

Abstract

The dCas9-based Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) interference (CRISPRi) gene regulation technique requires two components: a catalytically inactive Cas9 protein (dCas9) and a single-guide RNA that targets the gene of interest. This system is commonly activated by expressing dCas9 through an inducible gene promoter, but these inducers may affect cellular physiology, and accessibility and permeability of the inducer are limited in relevant model systems. Here, we have developed an alternative approach for CRISPRi activation in the clinical isolate

Indexed as

Bacterial ProteinsCRISPR-Cas SystemsGene Expression Regulation, BacterialGenes, ReporterPromoter Regions, GeneticStaphylococcus aureusVirulence FactorsClustered Regularly Interspaced Short Palindromic RepeatsHumansRNA, Guide, CRISPR-Cas SystemsVirulenceBacterial ProteinsRNA, Guide, CRISPR-Cas SystemsVirulence Factorsbacterial cell populationdCas9 toxicityinducer-free CRISPRiStaphylococcus aureus

Identifiers

PMID39162514
PMCPMC11448056

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.