Evidence map›Paper›PMID 39161731›Full record

ArticlePNAS nexus2024

Development of a novel bacterial production system for recombinant bioactive proteins completely free from endotoxin contamination.

Go Kamoshida, Daiki Yamaguchi, Yuki Kaya, Toshiki Yamakado, Kenta Yamashita, Moe Aoyagi, Saaya Nagai, Noriteru Yamada, Yu Kawagishi, Mizuki Sugano and 6 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in PNAS nexus, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors.

Go KamoshidaDepartment of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.ORCID https://orcid.org/0000-0001-6300-0911
Daiki YamaguchiLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.ORCID https://orcid.org/0009-0009-5581-7089
Yuki KayaLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.ORCID https://orcid.org/0009-0003-3926-7079
Toshiki YamakadoLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Kenta YamashitaLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Moe AoyagiLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Saaya NagaiLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Noriteru YamadaLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Yu KawagishiLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.
Mizuki SuganoDepartment of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Yoshiaki SakairiDepartment of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.
Mikako UenoDepartment of Intractable Diseases, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.
Norihiko TakemotoPathogenic Microbe Laboratory, Research Institute, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.
Yuji MoritaDepartment of Infection Control Science, Meiji Pharmaceutical University, 2-522-1 Noshio, Kiyose, Tokyo 204-8588, Japan.ORCID https://orcid.org/0000-0003-1371-8591
Yukihito IshizakaDepartment of Intractable Diseases, National Center for Global Health and Medicine, 1-21-1 Toyama, Shinjyuku-ku, Tokyo 162-8655, Japan.ORCID https://orcid.org/0000-0002-1863-6096
Kinnosuke YahiroLaboratory of Microbiology and Infection Control, Kyoto Pharmaceutical University, 5 Misasagi-Nakauchi-cho, Yamashina-ku, Kyoto 607-8414, Japan.ORCID https://orcid.org/0000-0002-9813-0780

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Endotoxins, or lipopolysaccharides (LPS), are potent immunostimulatory molecules of critical concern in bacterial recombinant protein expression systems. The gram-negative bacterium

Indexed as

Acinetobacter baumanniiendotoxinrecombinant protein expressionVHH

Identifiers

PMID39161731
PMCPMC11331542

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.