Evidence map›Paper›PMID 39158631›Full record

ReviewAnalytical and bioanalytical chemistry2024

Analysis of human biological samples using porous graphitic carbon columns and liquid chromatography-mass spectrometry: a review.

Taís Betoni Rodrigues, Ricardo Leal Cunha, Paulo Emílio Pereira Barci, Álvaro José Santos-Neto, Fernando Mauro Lanças

Erratum issuedAbstract readReview
PubMed Publisher
In one paragraph

Review in Analytical and bioanalytical chemistry, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Taís Betoni RodriguesLaboratory of Chromatography (CROMA), São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13560-970, Brazil. tais.betoni.rodrigues@usp.br.ORCID http://orcid.org/0000-0002-6973-8893
Ricardo Leal CunhaForensic Toxicology Laboratory, Scientific Police, São Cristóvão, Sergipe, 49100-000, Brazil.ORCID http://orcid.org/0000-0003-0444-7742
Paulo Emílio Pereira BarciLaboratory of Chromatography (CROMA), São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13560-970, Brazil.ORCID http://orcid.org/0000-0001-9612-6908
Álvaro José Santos-NetoLaboratory of Chromatography (CROMA), São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13560-970, Brazil.ORCID http://orcid.org/0000-0003-1719-8458
Fernando Mauro LançasLaboratory of Chromatography (CROMA), São Carlos Institute of Chemistry, University of São Paulo (USP), São Carlos, São Paulo, 13560-970, Brazil.ORCID http://orcid.org/0000-0002-8711-5905

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Liquid chromatography-mass spectrometry (LC-MS) has emerged as a powerful analytical technique for analyzing complex biological samples. Among various chromatographic stationary phases, porous graphitic carbon (PGC) columns have attracted significant attention due to their unique properties-such as the ability to separate both polar and non-polar compounds and their stability through all pH ranges and to high temperatures-besides the compatibility with LC-MS. This review discusses the applicability of PGC for SPE and separation in LC-MS-based analyses of human biological samples, highlighting the diverse applications of PGC-LC-MS in analyzing endogenous metabolites, pharmaceuticals, and biomarkers, such as glycans, proteins, oligosaccharides, sugar phosphates, and nucleotides. Additionally, the fundamental principles underlying PGC column chemistry and its advantages, challenges, and advances in method development are explored. This comprehensive review aims to provide researchers and practitioners with a valuable resource for understanding the capabilities and limitations of PGC columns in LC-MS-based analysis of human biological samples, thereby facilitating advancements in analytical methodologies and biomedical research.

Indexed as

GraphiteMass SpectrometryBiomarkersChromatography, LiquidHumansLiquid Chromatography-Mass SpectrometryPolysaccharidesPorosityProteinsSolid Phase ExtractionBiomarkersGraphitePolysaccharidesProteinsBioanalysisHuman biological samplesLC–MSPolar retention effect on graphitePorous graphitic carbon columns

Identifiers

PMID39158631

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.