Evidence map›Paper›PMID 39158391›Full record

ArticleThe Journal of infectious diseases2025

Ex Vivo Host Transcriptomics During Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans Infection of Peripheral Blood Mononuclear Cells From South African Volunteers.

Ronan M Doyle, Shichina Kannambath, Alan Pittman, Rene Goliath, Vinod Kumar, Graeme Meintjes, James Milburn, Mihai G Netea, Thomas S Harrison, Joseph N Jarvis and 1 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Journal of infectious diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ronan M DoyleDepartment of Clinical Research, London School of Hygiene and Tropical Medicine.ORCID 0000-0001-5001-1945
Shichina KannambathGenomics Facility, The Institute of Cancer Research.
Alan PittmanInstitute of Infection and Immunity, St George's University London, and Clinical Academic Group in Infection, St George's University Hospitals NHS Foundation Trust, London, United Kingdom.
Rene GoliathDepartment of Medicine and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa.
Vinod KumarBotswana Harvard Health Partnership, Gaborone.
Graeme MeintjesDepartment of Medicine and Institute of Infectious Disease and Molecular Medicine, University of Cape Town, South Africa.
James MilburnDepartment of Clinical Research, London School of Hygiene and Tropical Medicine.ORCID 0000-0001-9885-6103
Mihai G NeteaDepartment of Internal Medicine and Radboud Center for Infectious Diseases, Radboud University Medical Center, Nijmegen, The Netherlands.
Thomas S HarrisonInstitute of Infection and Immunity, St George's University London, and Clinical Academic Group in Infection, St George's University Hospitals NHS Foundation Trust, London, United Kingdom.
Joseph N JarvisDepartment of Clinical Research, London School of Hygiene and Tropical Medicine.
Tihana BicanicInstitute of Infection and Immunity, St George's University London, and Clinical Academic Group in Infection, St George's University Hospitals NHS Foundation Trust, London, United Kingdom.ORCID 0000-0002-2676-838X

Funding

National Institute for Health and Care Research RP-2017-08-ST2-012Strategic Award for Medical Mycology and Fungal Immunology 097377/Z/11/ZWellcome Trust
6 · The paper itself

Abstract

Cryptococcus neoformans, Cryptococcus gattii, and Candida albicans are opportunistic fungal pathogens associated with infections in immunocompromised hosts. Cryptococcal meningitis (CM) is the leading fungal cause of human immunodeficiency virus-related deaths globally, with the majority occurring in Africa. The human immune response to C albicans infection has been studied extensively in large genomics studies whereas cryptococcal infections, despite their severity, are comparatively understudied. Here we investigated the transcriptional response of immune cells after in vitro stimulation with in vitro C neoformans, C gattii, and C albicans infection of peripheral blood mononuclear cells collected from healthy South African volunteers. We found a lower transcriptional response to cryptococcal stimuli compared to C albicans and unique expression signatures from all 3 fungal stimuli. This work provides a starting point for further studies comparing the transcriptional signature of CM in immunocompromised patients, with the goal of identifying biomarkers of disease severity and possible novel treatment targets.

Indexed as

Candida albicansCandidiasisCryptococcosisCryptococcus gattiiCryptococcus neoformansLeukocytes, MononuclearTranscriptomeAdultFemaleGene Expression ProfilingHealthy VolunteersHost-Pathogen InteractionsHumansImmunocompromised HostMaleSouth AfricaCandida albicansCryptococcus gattiiCryptococcus neoformansfungal infectionimmune response

Identifiers

PMID39158391
PMCPMC11793030

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.