Evidence map›Paper›PMID 39157758›Full record

ArticlePlant direct2024

Impact of predictive selection of LbCas12a CRISPR RNAs upon on- and off-target editing rates in soybean.

Linda Rymarquis, Chenxi Wu, Diane Hohorst, Miguel Vega-Sanchez, Thomas E Mullen, Vijetha Vemulapalli, Douglas R Smith

Abstract read
In one paragraph

Article in Plant direct, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Linda RymarquisBayer Crop Science Chesterfield Missouri USA.ORCID https://orcid.org/0000-0003-0297-8371
Chenxi WuBayer Crop Science Chesterfield Missouri USA.
Diane HohorstBayer Crop Science Chesterfield Missouri USA.
Miguel Vega-SanchezBayer Crop Science Chesterfield Missouri USA.ORCID https://orcid.org/0000-0003-0128-2743
Thomas E MullenSeQure Dx Waltham Massachusetts USA.
Vijetha VemulapalliSeQure Dx Waltham Massachusetts USA.ORCID https://orcid.org/0009-0005-9460-102X
Douglas R SmithSeQure Dx Waltham Massachusetts USA.ORCID https://orcid.org/0000-0002-0401-7382

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized creating targeted genetic variation in crops. Although CRISPR enzymes have been reported to have high sequence-specificity, careful design of the editing reagents can also reduce unintended edits at highly homologous sites. This work details the first large-scale study of the heritability of on-target edits and the rate of edits at off-target sites in soybean (

Indexed as

genome editinggRNA designLbCas12a

Identifiers

PMID39157758
PMCPMC11328349

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.