Evidence map›Paper›PMID 39155517›Full record

ArticleOral diseases2025

Pleomorphic adenoma and carcinoma ex-pleomorphic adenoma tumorigenesis: A proteomic analysis.

Virgílio Gonzales Zanella, Sara Ferreira Dos Santos Costa, Lauren Frenzel Schuch, Emily Ferreira Salles Pilar, Adriana Franco Paes Leme, Jean Nunes Dos Santos, Syed Ali Khurram, Fatima Elalawy, Lynne Bingle, Fabio Daumas Nunes and 4 more

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PubMed Full text at PMC Publisher
In one paragraph

Article in Oral diseases, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors.

Virgílio Gonzales ZanellaDepartment of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.ORCID https://orcid.org/0000-0001-5721-9968
Sara Ferreira Dos Santos CostaDepartment of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.ORCID https://orcid.org/0000-0001-5150-9227
Lauren Frenzel SchuchDepartment of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.ORCID https://orcid.org/0000-0002-0993-936X
Emily Ferreira Salles PilarExperimental Research Unit, Hospital de Clínicas Porto Alegre, Porto Alegre, Brazil.ORCID https://orcid.org/0000-0003-3417-3586
Adriana Franco Paes LemeDepartment of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.ORCID https://orcid.org/0000-0001-7959-147X
Jean Nunes Dos SantosPostgraduation Program in Dentistry and Health, Federal University of Bahia, Salvador, Brazil.ORCID https://orcid.org/0000-0001-7225-5879
Syed Ali KhurramAcademic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.ORCID https://orcid.org/0000-0002-0378-9380
Fatima ElalawyAcademic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.
Lynne BingleAcademic Unit of Oral and Maxillofacial Medicine and Pathology, Department of Clinical Dentistry, University of Sheffield, Sheffield, UK.ORCID https://orcid.org/0000-0002-4587-9384
Fabio Daumas NunesDepartment of Oral and Maxillofacial Pathology, Dental School, University of São Paulo (USP), São Paulo, Brazil.ORCID https://orcid.org/0000-0002-7785-6785
Felipe Paiva FonsecaDepartment of Oral Surgery and Pathology, School of Dentistry, Federal University of Minas Gerais, Belo Horizonte, Brazil.ORCID https://orcid.org/0000-0002-6657-4547
Pablo Agustin VargasDepartment of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.ORCID https://orcid.org/0000-0003-1840-4911
Manoela Domingues MartinsDepartment of Pathology, School of Dentistry, Federal University of Rio Grande do Sul, Porto Alegre, Brazil.ORCID https://orcid.org/0000-0001-8662-5965
Vivian Petersen WagnerDepartment of Oral Diagnosis, Piracicaba Dental School, University of Campinas, Piracicaba, Brazil.ORCID https://orcid.org/0000-0002-1447-2135

Funding

Conselho Nacional de Desenvolvimento Científico e TecnológicoCoordenação de Aperfeiçoamento de Pessoal de Nível SuperiorFundação de Amparo à Pesquisa do Estado de Minas GeraisHospital de Clínicas de Porto Alegre
6 · The paper itself

Abstract

objectivesTo conduct a comprehensive proteomic analysis of normal salivary gland tissue, pleomorphic adenoma (PA), and carcinoma ex-pleomorphic adenoma (CXPA), and validate the proteomic findings using immunohistochemistry.

methodsSix normal salivary gland tissues, seven PA and seven CXPA samples underwent laser microdissection followed by liquid chromatography coupled to mass spectrometry. Protein identification and quantification were performed using MaxQuant software. Statistical analysis and functional enrichment were conducted using the Perseus platform and STRING tool, respectively. Immunohistochemistry was used for validation.

resultsComparative proteomic analysis revealed 2680 proteins across the three tissue types, with 799 significantly altered between groups. Translocation protein SEC63 homolog, Annexin A6 and Biglycan were up-regulated in CXPA compared to PA. Decorin was markedly up-regulated in both PA and CXPA compared to normal salivary gland (log

conclusionsProteomic analysis identified differential protein expression patterns associated with malignant transformation of PA into CXPA. Findings indicate a crucial role for extracellular matrix proteins, specifically Biglycan and Decorin, in the tumorigenic progression of PA and CXPA.

Indexed as

Adenoma, PleomorphicCarcinomaSalivary Gland NeoplasmsAdultBiglycanCarcinogenesisCell Transformation, NeoplasticDecorinFemaleHumansImmunohistochemistryMaleMiddle AgedProteomicsSalivary GlandsBiglycanDecorincarcinoma ex‐pleomorphic adenomaglandular and epithelialhead and neck neoplasmsproteomicrare diseases

Identifiers

PMID39155517
PMCPMC12021308

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.