ArticleBMC veterinary research2024
Cortisone in saliva of pigs: validation of a new assay and changes after thermal stress.
Article in BMC veterinary research, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.
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The trial behind it
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Who cites it
4 citing papers in PubMed.
- The Salivary Microbiota-Host Nexus: Unraveling Opportunities for Non-Invasive Monitoring of Health and Productivity in Farm Animals.Animals : an open access journal from MDPI · 2026Review
- Salivary responses to an experimental activation of the HPA axis in pigs: Effects of ACTH on analytes related to stress, immune system, inflammation, redox status and metabolism.Veterinary and animal science · 2026Article
- Effect of Preweaning Socialization on Postweaning Biomarkers of Stress, Inflammation, Immunity and Metabolism in Saliva and Serum of Iberian Piglets.Animals : an open access journal from MDPI · 2025Article
- Hair hormone profiling as a non-invasive diagnostic approach for assessing long-term endocrine status and productivity in Hereford bulls.Veterinary world · 2025Article
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundCortisone is derived from cortisol through the action of the enzyme 11β-hydroxysteroid dehydrogenase type II, and it has gained importance in recent years as a biomarker of stress. This study aimed to develop and validate an assay for the measurement of cortisone in pig saliva and evaluate whether its concentration varies in stressful situations. For this purpose, a specific immunoassay was developed and validated analytically, and a study was performed to evaluate whether cortisone concentrations in saliva can vary under heat stress conditions.
resultsThe assay proved to be accurate, reliable, and sensitive for the measurement of cortisone in pig saliva. The limit of detection of the assay was set at 0.006 ng/ml, and the lower limit of quantification was 0.023 ng/ml. It also correlated significantly with the results obtained by LC‒MS/MS (P = 0.003; r = 0.64). In addition, the cortisone concentration in animals subjected to prolonged heat stress decreased significantly 15 days after treatment (P < 0.0001).
conclusionsAccording to these results, cortisone measured by this assay could be used as a tool for the non-invasive evaluation of thermal stress in pig saliva.
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