Evidence map›Paper›PMID 39155366›Full record

ArticleVirology journal2024

Development and validation of a rapid five-minute nucleic acid extraction method for respiratory viruses.

Yu Wang, Yuanyuan Huang, Yuqing Peng, Qinglin Cao, Wenkuan Liu, Zhichao Zhou, Guangxin Xu, Lei Li, Rong Zhou

Abstract readValidation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Virology journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Review
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Yu WangGuangzhou National Laboratory, Guangzhou, China.
Yuanyuan HuangGuangzhou National Laboratory, Guangzhou, China.
Yuqing PengGuangzhou National Laboratory, Guangzhou, China.
Qinglin CaoGuangzhou National Laboratory, Guangzhou, China.
Wenkuan LiuState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Zhichao ZhouState Key Laboratory of Respiratory Disease, National Clinical Research Center for Respiratory Disease, Guangzhou Institute of Respiratory Health, the First Affiliated Hospital of Guangzhou Medical University, Guangzhou Medical University, Guangzhou, China.
Guangxin XuGuangzhou National Laboratory, Guangzhou, China.
Lei LiSchool of Pharmacy, Tongji Medical College, Huazhong University of Science of Technology, Wuhan, China. 918666035@qq.com.
Rong ZhouGuangzhou National Laboratory, Guangzhou, China. zhou_rong@gzlab.ac.cn.

Funding

Emergency Key Program of Guangzhou Laboratory EKPG21-13Research and Development Project in the Key Areas of Guangdong Province 2022B1111020003
6 · The paper itself

Abstract

backgroundThe rapid transmission and high pathogenicity of respiratory viruses significantly impact the health of both children and adults. Extracting and detecting their nucleic acid is crucial for disease prevention and treatment strategies. However, current extraction methods are laborious and time-consuming and show significant variations in nucleic acid content and purity among different kits, affecting detection sensitivity and efficiency. Our aim is to develop a novel method that reduces extraction time, simplifies operational steps, and ensures high-quality acquisition of respiratory viral nucleic acid.

methodsWe extracted respiratory syncytial virus (RSV) nucleic acid using reagents with different components and analyzed cycle threshold (Ct) values via quantitative real-time polymerase chain reaction (qRT-PCR) to optimize and validate the novel lysis and washing solution. The performance of this method was compared against magnetic bead, spin column, and precipitation methods for extracting nucleic acid from various respiratory viruses. The clinical utility of this method was confirmed by comparing it to the standard magnetic bead method for extracting clinical specimens of influenza A virus (IAV).

resultsThe solution, composed of equal parts glycerin and ethanol (50% each), offers an innovative washing approach that achieved comparable efficacy to conventional methods in a single abbreviated cycle. When combined with our A Plus lysis solution, our novel five-minute nucleic acid extraction (FME) method for respiratory viruses yielded superior RNA concentrations and purity compared to traditional methods. FME, when used with a universal automatic nucleic acid extractor, demonstrated similar efficiency as various conventional methods in analyzing diverse concentrations of respiratory viruses. In detecting respiratory specimens from 525 patients suspected of IAV infection, the FME method showed an equivalent detection rate to the standard magnetic bead method, with a total coincidence rate of 95.43% and a kappa statistic of 0.901 (P < 0.001).

conclusionsThe FME developed in this study enables the rapid and efficient extraction of nucleic acid from respiratory samples, laying a crucial foundation for the implementation of expedited molecular diagnosis.

Indexed as

Real-Time Polymerase Chain ReactionRNA, ViralHumansInfluenza A virusInfluenza, HumanMolecular Diagnostic TechniquesRespiratory Tract InfectionsSpecimen HandlingTime FactorsVirusesRNA, ViralFive-minute nucleic acid extractionGlycerin and ethanolRespiratory viruses

Identifiers

PMID39155366
PMCPMC11331601

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.