ArticleMethods in enzymology2024
Linking biosynthetic genes to natural products using inverse stable isotopic labeling (InverSIL).
Article in Methods in enzymology, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Article
- Inverse stable isotopic labeling for natural product characterization and discovery.Natural product reports · 2026Review
- Inverse stable isotope labeling (InverSIL) links predicted catecholate siderophore gene clusters to their products in diverse bacteria.bioRxiv : the preprint server for biology · 2025Article
- Inverse stable isotope probing-metabolomics (InverSIP) identifies an iron acquisition system in a methane-oxidizing bacterial community.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Glutathione in the Biosynthesis of Glutazoline Teredinibactins.Organic letters · 2025Article
- Detection of Inverse Stable Isotopic Labeling in Untargeted Metabolomic Data.Analytical chemistry · 2024Article
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2 authors.
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Abstract
The sequencing of microbial genomes has far outpaced their functional annotation. Stable isotopic labeling can be used to link biosynthetic genes with their natural products; however, the availability of the required isotopically substituted precursors can limit the accessibility of this approach. Here, we describe a method for using inverse stable isotopic labeling (InverSIL) to link biosynthetic genes with their natural products. With InverSIL, a microbe is grown on an isotopically substituted medium to create a fully substituted culture, and subsequently, the incorporation of precursors of natural isotopic abundance can be tracked by mass spectrometry. This eliminates issues with isotopically substituted precursor availability. We demonstrate the utility of this approach by linking a luxI-type acyl-homoserine lactone synthase gene in a bacterium that grows on methanol with its quorum sensing signal products. In the future, InverSIL can also be used to link biosynthetic gene clusters hypothesized to produce siderophores with their natural products.
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