Evidence map›Paper›PMID 39155022›Full record

ArticleJournal of endodontics2024

Antimicrobial Silk Fibroin Methacrylated Scaffolds for Regenerative Endodontics.

Ramyasaketha Narayanam, Lais M Cardoso, Alexandre H Dos Reis-Prado, Ana Beatriz G de Carvalho, Caroline Anselmi, Abdel H Mahmoud, J Christopher Fenno, Renan Dal-Fabbro, Marco C Bottino

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of endodontics, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Review
  2. Review
  3. Review
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Ramyasaketha NarayanamDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
Lais M CardosoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP)-Araraquara School of Dentistry, Araraquara, São Paulo, Brazil.
Alexandre H Dos Reis-PradoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Restorative Dentistry, Minas Gerais Federal University (UFMG)-School of Dentistry, Belo Horizonte, Minas Gerais, Brazil.
Ana Beatriz G de CarvalhoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP)-Sao Jose dos Campos School of Dentistry, São José dos Campos, São Paulo, Brazil.
Caroline AnselmiDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Morphology and Pediatric Dentistry, São Paulo State University (UNESP)-Araraquara School of Dentistry, Araraquara, São Paulo, Brazil.
Abdel H MahmoudDepartment of Oral Biology and Pathology, Stony Brook University-School of Dental Medicine, Stony Brook, New York.
J Christopher FennoDepartment of Biologic and Materials Sciences & Prosthodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
Renan Dal-FabbroDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan.
Marco C BottinoDepartment of Cariology, Restorative Sciences and Endodontics, University of Michigan-School of Dentistry, Ann Arbor, Michigan; Department of Biomedical Engineering, College of Engineering, University of Michigan, Ann Arbor, Michigan. Electronic address: mbottino@umich.edu.

Funding

Personalized Strategies for Periodontal Tissue Regeneration - A Converged Biofabrication ApproachR01DE031476 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI Marco C Bottino · 2022 to 2026
$2.6M
NOVEL THERAPEUTICS TO TREAT NECROTIC TEETH: THE COMBINATORIAL EFFECT OF A 3D DRUG DELIVERY SYSTEM AND SPATIALLY DESIGNED STEM CELL NICHESR01DE026578 · NIDCR · UNIVERSITY OF MICHIGAN AT ANN ARBOR · PI BOTTINO, MARCO C · 2017 to 2021
$1.7M
NIDCR NIH HHS R01 DE026578NIDCR NIH HHS R01 DE031476
6 · The paper itself

Abstract

introductionRecognizing the necessity of novel disinfection strategies for improved bacterial control to ultimately favor tissue regeneration, this study developed and characterized antibiotics-laden silk fibroin methacrylated (SilkMA) scaffolds for regenerative endodontics.

methodsSilkMA-based solutions (10% w/v) containing Clindamycin (CLI) or Tinidazole (TIN) (0 - control; 5, 10, or 15% w/w) or the combination of both drugs (BiMix CLI/TIN 10%) were electrospun and photocrosslinked. Morphology and composition were assessed using scanning electron microscopy and Fourier-transform infrared spectroscopy. Additionally, swelling and degradation profiles were also determined. Cytotoxicity was evaluated in stem cells from apical papilla. Antibacterial efficacy was tested using direct and indirect contact assays against Aggregatibacter actinomycetemcomitans/Aa, Actinomyces naeslundii/An, Enterococcus faecalis/Ef, and Fusobacterium nucleatum/Fn. E. faecalis biofilm inhibition on dentin discs was specifically evaluated for BiMix-laden scaffolds. Data were statistically analyzed with a significance level of 5%.

resultsScanning electron microscopy revealed that all scaffolds had similar characteristics, including fiber morphology and bead absence. Fourier-transform infrared spectroscopy showed the incorporation of CLI and TIN into the fibers and in BiMix scaffolds. Antibiotic-laden scaffolds exhibited lower swelling capacity than the control and were degraded entirely after 45 days. Scaffolds laden with CLI, TIN, or BiMix throughout all time points did not reduce stem cells from apical papilla's viability. CLI-laden scaffolds inhibited the growth of Aa, An, and Ef, while TIN-laden scaffolds inhibited Fn growth. BiMix-laden scaffolds significantly inhibited Aa, An, Ef, and Fn in direct contact, and their aliquots inhibited An and Fn through indirect contact, with additional biofilm inhibition against Ef.

conclusionsBiMix-laden SilkMA scaffolds are cytocompatible and exhibit antimicrobial effects against endodontic pathogens, indicating their therapeutic potential as a drug delivery system for regenerative endodontics.

Indexed as

Enterococcus faecalisFibroinsRegenerative EndodonticsTissue ScaffoldsActinomycesAggregatibacter actinomycetemcomitansAnti-Bacterial AgentsBiofilmsClindamycinFusobacterium nucleatumHumansMicroscopy, Electron, ScanningSpectroscopy, Fourier Transform InfraredStem CellsAnti-Bacterial AgentsClindamycinFibroinsClindamycinregenerative endodonticssilktinidazoletissue engineering

Identifiers

PMID39155022
PMCPMC13033333

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.