Evidence map›Paper›PMID 39154341›Full record

ArticleCell reports2024

The Chlamydia effector IncE employs two short linear motifs to reprogram host vesicle trafficking.

Khavong Pha, Kathleen Mirrashidi, Jessica Sherry, Cuong Joseph Tran, Clara M Herrera, Eleanor McMahon, Cherilyn A Elwell, Joanne N Engel

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Inclusion Membrane Proteins ofMicroorganisms · 2026
    Review
  2. Article
  3. Article
  4. Review
  5. ThebioRxiv : the preprint server for biology · 2025
    Article
  6. Pathogen-induced rerouting of host membrane trafficking.Current opinion in cell biology · 2025
    Review
  7. Article
  8. Cell signaling inFrontiers in cellular and infection microbiology · 2025
    Review
4 · The record

Corrections and comments

  • Update of
    The2024
5 · Who and what money

Authors and funding

8 authors.

Khavong PhaDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Kathleen MirrashidiDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Jessica SherryDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Cuong Joseph TranDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Clara M HerreraDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Eleanor McMahonDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA.
Cherilyn A ElwellDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: cherilyn.elwell@ucsf.edu.
Joanne N EngelDepartment of Medicine, University of California, San Francisco, San Francisco, CA 94143, USA; Department of Microbiology and Immunology, University of California, San Francisco, San Francisco, CA 94143, USA. Electronic address: jengel@medicine.ucsf.edu.

Funding

Dissecting the role of the Inclusion membrane protein IncE, a master multi-tasking scaffolding protein, in the pathogenesis of Chlamydia trachomatis infectionsR01AI163526 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI Joanne N. Engel · 2022 to 2026
$3.3M
Decoding the Chlamydia inclusion membrane protein-host protein interactomeR01AI122747 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ENGEL, JOANNE N., KAAKE, ROBYN MIDORI · 2016 to 2020
$3.1M
Inclusion membrane protein (Inc) modulation of the innate immune response to Chlamydia trachomatisR56AI152526 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI ENGEL, JOANNE N., SWANEY, DANIELLE L · 2020 to 2020
$803k
Remodeling of the Host Ubiquitin Landscape by Chlamydia trachomatisF32AI133902 · NIAID · UNIVERSITY OF CALIFORNIA, SAN FRANCISCO · PI PHA, KHAVONG · 2017 to 2019
$179k
NIAID NIH HHS F32 AI133902NIAID NIH HHS R01 AI122747NIAID NIH HHS R01 AI163526NIAID NIH HHS R56 AI152526
6 · The paper itself

Abstract

Chlamydia trachomatis, a leading cause of bacterial sexually transmitted infections, creates a specialized intracellular replicative niche by translocation and insertion of a diverse array of effectors (Incs [inclusion membrane proteins]) into the inclusion membrane. Here, we characterize IncE, a multifunctional Inc that encodes two non-overlapping short linear motifs (SLiMs) within its short cytosolic C terminus. The proximal SLiM, by mimicking just a small portion of an R-N-ethylmaleimide-sensitive factor adaptor protein receptor (SNARE) motif, binds and recruits syntaxin (STX)7- and STX12-containing vesicles to the inclusion. The distal SLiM mimics the sorting nexin (SNX)5 and SNX6 cargo binding site to recruit SNX6-containing vesicles to the inclusion. By simultaneously binding two distinct vesicle classes, IncE brings these vesicles in close apposition with each other at the inclusion to facilitate C. trachomatis intracellular development. Our work suggests that Incs may have evolved SLiMs to enable rapid evolution in a limited protein space to disrupt host cell processes.

Indexed as

Bacterial ProteinsChlamydia trachomatisAmino Acid MotifsHeLa CellsHumansProtein BindingProtein TransportQa-SNARE ProteinsSorting NexinsBacterial ProteinsQa-SNARE ProteinsSorting NexinsChlamydia trachomatisCP: Microbiologyhost-pathogen interactionsinclusion membrane proteinintracellular bacteriamicrobial pathogenesisshort linear motifSNARESNARE proteinsorting nexinsyntaxinvesicular trafficking

Identifiers

PMID39154341
PMCPMC12108946

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.