Electrochemical detection of anti-tissue transglutaminase antibody using quantum dots-doped polypyrrole-modified electrode.

Cristina Dumitriu, Andreea Madalina Pandele, Mihaela Vasilica Mîndroiu, Oana-Andreea Lazar, Alina Popp, Marius Enachescu, George-Octavian Buica

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Article in Mikrochimica acta, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1 citing paper in PubMed.

Anti-Tissue-Transglutaminase IgA Antibodies Presence Determination Using Electrochemical Square Wave Voltammetry and Modified Electrodes Based on Polypyrrole and Quantum Dots.Biosensors · 2025
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Authors and funding

7 authors.

Cristina DumitriuNational University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.

Andreea Madalina PandeleNational University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.

Mihaela Vasilica MîndroiuNational University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.

Oana-Andreea LazarCenter for Surface Science and Nanotechnology, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.

Alina PoppNational Institute for Mother and Child Health "Alessandrescu-Rusescu", 120 Lacul Tei Boulevard, Sector 2, 020395, Bucharest, Romania.

Marius EnachescuCenter for Surface Science and Nanotechnology, National University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania.

George-Octavian BuicaNational University of Science and Technology Politehnica Bucharest, 313 Splaiul Independentei, Sector 6, 060042, Bucharest, Romania. buica_george@yahoo.com.

Funding

a grant of the Ministry of Research, Inovation and Digitalization, CCCDI-UEFISCDI, within PNCDI III PN-III-P2-2.1-PED-2021-4405

6 · The paper itself

Abstract

A nanohybrid-modified glassy carbon electrode based on conducting polypyrrole doped with carbon quantum dots (QDs) was developed and used for the electrochemical detection of anti-tissue transglutaminase (anti-tTG) antibodies. To improve the polypyrrole conductivity, carrier mobility, and carrier concentration, four types of carbon nanoparticles were tested. Furthermore, a polypyrrole-modified electrode doped with QDs was functionalized with a PAMAM dendrimer and transglutaminase 2 protein by cross-linking with N-hydroxysuccinimide (NHS)/N-(3-dimethylaminopropyl)-N'-ethylcarbodiimide hydrochloride (EDC). The steps of electrode surface modification were surveyed via electrochemical measurements (differential pulse voltammetry (DPV), impedance spectroscopy, and X-ray photoelectron spectroscopy (XPS)). The surface characteristics were observed by scanning electron microscopy (SEM), Fourier transform infrared (FTIR) spectroscopy, and contact angle measurements. The obtained modified electrode exhibited good stability and repeatability. DPV between - 0.1 and 0.6 V (vs. Ag/AgCl 3 M KCl reference electrode) was used to evaluate the electrochemical alterations that occur after the antibody interacts with the antigen (transglutaminase 2 protein), for which the limit of detection was 0.79 U/mL. Without the use of a secondary label, (anti-tTG) antibodies may be detected at low concentrations because of these modified electrode features.

Indexed as

DendrimersProtein Glutamine gamma Glutamyltransferase 2PyrrolesQuantum DotsTransglutaminasesAntibodiesBiosensing TechniquesCarbonElectrochemical TechniquesElectrodesGTP-Binding ProteinsHumansPolymersAntibodiesCarbonDendrimersGTP-Binding ProteinsPAMAM StarburstPolymerspolypyrroleProtein Glutamine gamma Glutamyltransferase 2PyrrolesTransglutaminasesAnti-tTG antibody detectionCarbon quantum dotsDifferential pulse voltammetryElectroanalysisModified glassy carbon electrodePolypyrrole-modified electrode

Identifiers

PMID39153033

DOI10.1007/s00604-024-06620-w

PMCPMC11330391

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